Human sgRNA library Brunello in lentiCRISPRv2 Citations (68)
Originally described in: Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PubMed Journal
Articles Citing Human sgRNA library Brunello in lentiCRISPRv2
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| Exosome-mediated miR-146a transfer suppresses type I interferon response and facilitates EV71 infection. Fu Y, Zhang L, Zhang F, Tang T, Zhou Q, Feng C, Jin Y, Wu Z. PLoS Pathog. 2017 Sep 14;13(9):e1006611. doi: 10.1371/journal.ppat.1006611. eCollection 2017 Sep. PubMed |
| Next-Generation Sequencing of Genome-Wide CRISPR Screens. Yau EH, Rana TM. Methods Mol Biol. 2018;1712:203-216. doi: 10.1007/978-1-4939-7514-3_13. PubMed |
| Pooled Lentiviral-Delivery Genetic Screens. Piccioni F, Younger ST, Root DE. Curr Protoc Mol Biol. 2018 Jan 16;121:32.1.1-32.1.21. doi: 10.1002/cpmb.52. PubMed |
Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities.
Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG.
Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8.
PubMed
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| Multiple-gene targeting and mismatch tolerance can confound analysis of genome-wide pooled CRISPR screens. Fortin JP, Tan J, Gascoigne KE, Haverty PM, Forrest WF, Costa MR, Martin SE. Genome Biol. 2019 Jan 25;20(1):21. doi: 10.1186/s13059-019-1621-7. PubMed |
| MHC class II proteins mediate cross-species entry of bat influenza viruses. Karakus U, Thamamongood T, Ciminski K, Ran W, Gunther SC, Pohl MO, Eletto D, Jeney C, Hoffmann D, Reiche S, Schinkothe J, Ulrich R, Wiener J, Hayes MGB, Chang MW, Hunziker A, Yanguez E, Aydillo T, Krammer F, Oderbolz J, Meier M, Oxenius A, Halenius A, Zimmer G, Benner C, Hale BG, Garcia-Sastre A, Beer M, Schwemmle M, Stertz S. Nature. 2019 Mar;567(7746):109-112. doi: 10.1038/s41586-019-0955-3. Epub 2019 Feb 20. PubMed |
| The TP53 Apoptotic Network Is a Primary Mediator of Resistance to BCL2 Inhibition in AML Cells. Nechiporuk T, Kurtz SE, Nikolova O, Liu T, Jones CL, D'Alessandro A, Culp-Hill R, d'Almeida A, Joshi SK, Rosenberg M, Tognon CE, Danilov AV, Druker BJ, Chang BH, McWeeney SK, Tyner JW. Cancer Discov. 2019 Jul;9(7):910-925. doi: 10.1158/2159-8290.CD-19-0125. Epub 2019 May 2. PubMed |
| SLC1A3 contributes to L-asparaginase resistance in solid tumors. Sun J, Nagel R, Zaal EA, Ugalde AP, Han R, Proost N, Song JY, Pataskar A, Burylo A, Fu H, Poelarends GJ, van de Ven M, van Tellingen O, Berkers CR, Agami R. EMBO J. 2019 Oct 4;38(21):e102147. doi: 10.15252/embj.2019102147. Epub 2019 Sep 16. PubMed |
| Delete_Genetic screens in isogenic mammalian cell lines without single cell cloning. DeWeirdt PC, Sangree AK, Hanna RE, Sanson KR, Hegde M, Strand C, Persky NS, Doench JG. Nat Commun. 2020 Feb 6;11(1):752. doi: 10.1038/s41467-020-14620-6. PubMed |
| Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus. Das A, Barrientos R, Shiota T, Madigan V, Misumi I, McKnight KL, Sun L, Li Z, Meganck RM, Li Y, Kaluzna E, Asokan A, Whitmire JK, Kapustina M, Zhang Q, Lemon SM. Nat Microbiol. 2020 Sep;5(9):1069-1078. doi: 10.1038/s41564-020-0727-8. Epub 2020 May 25. PubMed |
| The miR-26b-5p/KPNA2 Axis Is an Important Regulator of Burkitt Lymphoma Cell Growth. Niu F, Kazimierska M, Nolte IM, Terpstra MM, de Jong D, Koerts J, van der Sluis T, Rutgers B, O'Connell RM, Kok K, van den Berg A, Dzikiewicz-Krawczyk A, Kluiver J. Cancers (Basel). 2020 Jun 4;12(6). pii: cancers12061464. doi: 10.3390/cancers12061464. PubMed |
| Genome-wide CRISPR synthetic lethality screen identifies a role for the ADP-ribosyltransferase PARP14 in DNA replication dynamics controlled by ATR. Dhoonmoon A, Schleicher EM, Clements KE, Nicolae CM, Moldovan GL. Nucleic Acids Res. 2020 Jul 27;48(13):7252-7264. doi: 10.1093/nar/gkaa508. PubMed |
| Cotargeting of XPO1 Enhances the Antileukemic Activity of Midostaurin and Gilteritinib in Acute Myeloid Leukemia. Brinton LT, Sher S, Williams K, Canfield D, Orwick S, Wasmuth R, Cempre C, Skinner J, Lehman A, Blachly JS, Byrd JC, Lapalombella R. Cancers (Basel). 2020 Jun 14;12(6). pii: cancers12061574. doi: 10.3390/cancers12061574. PubMed |
| Ribosome Recycling by ABCE1 Links Lysosomal Function and Iron Homeostasis to 3' UTR-Directed Regulation and Nonsense-Mediated Decay. Zhu X, Zhang H, Mendell JT. Cell Rep. 2020 Jul 14;32(2):107895. doi: 10.1016/j.celrep.2020.107895. PubMed |
| Elucidation of remdesivir cytotoxicity pathways through genome-wide CRISPR-Cas9 screening and transcriptomics. Akinci E, Cha M, Lin L, Yeo G, Hamilton MC, Donahue CJ, Bermudez-Cabrera HC, Zanetti LC, Chen M, Barkal SA, Khowpinitchai B, Chu N, Velimirovic M, Jodhani R, Fife JD, Sovrovic M, Cole PA, Davey RA, Cassa CA, Sherwood RI. bioRxiv. 2020 Aug 28. doi: 10.1101/2020.08.27.270819. PubMed |
| FBXO11 is a candidate tumor suppressor in the leukemic transformation of myelodysplastic syndrome. Schieber M, Marinaccio C, Bolanos LC, Haffey WD, Greis KD, Starczynowski DT, Crispino JD. Blood Cancer J. 2020 Oct 6;10(10):98. doi: 10.1038/s41408-020-00362-7. PubMed |
| A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming. Han J, LaVigne CA, Jones BT, Zhang H, Gillett F, Mendell JT. Science. 2020 Dec 18;370(6523). pii: science.abc9546. doi: 10.1126/science.abc9546. Epub 2020 Nov 12. PubMed |
| Identification of regulators of poly-ADP-ribose polymerase inhibitor response through complementary CRISPR knockout and activation screens. Clements KE, Schleicher EM, Thakar T, Hale A, Dhoonmoon A, Tolman NJ, Sharma A, Liang X, Imamura Kawasawa Y, Nicolae CM, Wang HG, De S, Moldovan GL. Nat Commun. 2020 Nov 30;11(1):6118. doi: 10.1038/s41467-020-19961-w. PubMed |
Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.
Neggers JE, Paolella BR, Asfaw A, Rothberg MV, Skipper TA, Yang A, Kalekar RL, Krill-Burger JM, Dharia NV, Kugener G, Kalfon J, Yuan C, Dumont N, Gonzalez A, Abdusamad M, Li YY, Spurr LF, Wu WW, Durbin AD, Wolpin BM, Piccioni F, Root DE, Boehm JS, Cherniack AD, Tsherniak A, Hong AL, Hahn WC, Stegmaier K, Golub TR, Vazquez F, Aguirre AJ.
Cell Rep. 2020 Dec 15;33(11):108493. doi: 10.1016/j.celrep.2020.108493.
PubMed
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| CRISPR Screening of CAR T Cells and Cancer Stem Cells Reveals Critical Dependencies for Cell-Based Therapies. Wang D, Prager BC, Gimple RC, Aguilar B, Alizadeh D, Tang H, Lv D, Starr R, Brito A, Wu Q, Kim LJY, Qiu Z, Lin P, Lorenzini MH, Badie B, Forman SJ, Xie Q, Brown CE, Rich JN. Cancer Discov. 2021 May;11(5):1192-1211. doi: 10.1158/2159-8290.CD-20-1243. Epub 2020 Dec 16. PubMed |
| Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD. Hewitt G, Borel V, Segura-Bayona S, Takaki T, Ruis P, Bellelli R, Lehmann LC, Sommerova L, Vancevska A, Tomas-Loba A, Zhu K, Cooper C, Fugger K, Patel H, Goldstone R, Schneider-Luftman D, Herbert E, Stamp G, Brough R, Pettitt S, Lord CJ, West SC, Ahel I, Ahel D, Chapman JR, Deindl S, Boulton SJ. Mol Cell. 2021 Feb 18;81(4):767-783.e11. doi: 10.1016/j.molcel.2020.12.006. Epub 2020 Dec 16. PubMed |
| Functional Genomics Identify Distinct and Overlapping Genes Mediating Resistance to Different Classes of Heterobifunctional Degraders of Oncoproteins. Shirasaki R, Matthews GM, Gandolfi S, de Matos Simoes R, Buckley DL, Raja Vora J, Sievers QL, Bruggenthies JB, Dashevsky O, Poarch H, Tang H, Bariteau MA, Sheffer M, Hu Y, Downey-Kopyscinski SL, Hengeveld PJ, Glassner BJ, Dhimolea E, Ott CJ, Zhang T, Kwiatkowski NP, Laubach JP, Schlossman RL, Richardson PG, Culhane AC, Groen RWJ, Fischer ES, Vazquez F, Tsherniak A, Hahn WC, Levy J, Auclair D, Licht JD, Keats JJ, Boise LH, Ebert BL, Bradner JE, Gray NS, Mitsiades CS. Cell Rep. 2021 Jan 5;34(1):108532. doi: 10.1016/j.celrep.2020.108532. PubMed |
| Accelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing. Mattsson J, Ekdahl L, Junghus F, Ajore R, Erlandsson E, Niroula A, Pertesi M, Frendeus B, Teige I, Nilsson B. Nat Commun. 2021 Feb 24;12(1):1277. doi: 10.1038/s41467-021-21518-4. PubMed |
| Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2. Baggen J, Persoons L, Vanstreels E, Jansen S, Van Looveren D, Boeckx B, Geudens V, De Man J, Jochmans D, Wauters J, Wauters E, Vanaudenaerde BM, Lambrechts D, Neyts J, Dallmeier K, Thibaut HJ, Jacquemyn M, Maes P, Daelemans D. Nat Genet. 2021 Apr;53(4):435-444. doi: 10.1038/s41588-021-00805-2. Epub 2021 Mar 8. PubMed |
| SAM homeostasis is regulated by CFIm-mediated splicing of MAT2A. Scarborough AM, Flaherty JN, Hunter OV, Liu K, Kumar A, Xing C, Tu BP, Conrad NK. Elife. 2021 May 5;10. pii: 64930. doi: 10.7554/eLife.64930. PubMed |
| Receptor Switching in Newly Evolved Adeno-associated Viruses. Havlik LP, Das A, Mietzsch M, Oh DK, Ark J, McKenna R, Agbandje-McKenna M, Asokan A. J Virol. 2021 Sep 9;95(19):e0058721. doi: 10.1128/JVI.00587-21. Epub 2021 Sep 9. PubMed |
| Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer. Vichas A, Riley AK, Nkinsi NT, Kamlapurkar S, Parrish PCR, Lo A, Duke F, Chen J, Fung I, Watson J, Rees M, Gabel AM, Thomas JD, Bradley RK, Lee JK, Hatch EM, Baine MK, Rekhtman N, Ladanyi M, Piccioni F, Berger AH. Nat Commun. 2021 Aug 9;12(1):4789. doi: 10.1038/s41467-021-24841-y. PubMed |
| Genome-wide CRISPR-Cas9 screen identifies rationally designed combination therapies for CRLF2-rearranged Ph-like ALL. Sasaki K, Yamauchi T, Semba Y, Nogami J, Imanaga H, Terasaki T, Nakao F, Akahane K, Inukai T, Verhoeyen E, Akashi K, Maeda T. Blood. 2022 Feb 3;139(5):748-760. doi: 10.1182/blood.2021012976. PubMed |
| Identification of Drug Resistance Genes Using a Pooled Lentiviral CRISPR/Cas9 Screening Approach. Kerek EM, Cromwell CR, Hubbard BP. Methods Mol Biol. 2021;2381:227-242. doi: 10.1007/978-1-0716-1740-3_13. PubMed |
| Considerations and practical implications of performing a phenotypic CRISPR/Cas survival screen. Ashoti A, Limone F, van Kranenburg M, Alemany A, Baak M, Vivie J, Piccioni F, Dijkers PF, Creyghton M, Eggan K, Geijsen N. PLoS One. 2022 Feb 17;17(2):e0263262. doi: 10.1371/journal.pone.0263262. eCollection 2022. PubMed |
| VPS29 Exerts Opposing Effects on Endocytic Viral Entry. Poston D, Weisblum Y, Hobbs A, Bieniasz PD. mBio. 2022 Apr 26;13(2):e0300221. doi: 10.1128/mbio.03002-21. Epub 2022 Mar 1. PubMed |
| Molecular Virology of SARS-CoV-2 and Related Coronaviruses. Kung YA, Lee KM, Chiang HJ, Huang SY, Wu CJ, Shih SR. Microbiol Mol Biol Rev. 2022 Jun 15;86(2):e0002621. doi: 10.1128/mmbr.00026-21. Epub 2022 Mar 28. PubMed |
| CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity. Bernareggi D, Xie Q, Prager BC, Yun J, Cruz LS, Pham TV, Kim W, Lee X, Coffey M, Zalfa C, Azmoon P, Zhu H, Tamayo P, Rich JN, Kaufman DS. Nat Commun. 2022 Apr 7;13(1):1899. doi: 10.1038/s41467-022-29469-0. PubMed |
| Cellular abundance of sodium phosphate cotransporter SLC20A1/PiT1 and phosphate uptake are controlled post-transcriptionally by ESCRT. Zechner C, Henne WM, Sathe AA, Xing C, Hernandez G, Sun S, Cheong MC. J Biol Chem. 2022 Jun;298(6):101945. doi: 10.1016/j.jbc.2022.101945. Epub 2022 Apr 18. PubMed |
| Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Israeli M, Finkel Y, Yahalom-Ronen Y, Paran N, Chitlaru T, Israeli O, Cohen-Gihon I, Aftalion M, Falach R, Rotem S, Elia U, Nemet I, Kliker L, Mandelboim M, Beth-Din A, Israely T, Cohen O, Stern-Ginossar N, Bercovich-Kinori A. Nat Commun. 2022 Apr 25;13(1):2237. doi: 10.1038/s41467-022-29896-z. PubMed |
| Genomewide CRISPR knockout screen identified PLAC8 as an essential factor for SADS-CoVs infection. Tse LV, Meganck RM, Araba KC, Yount BL, Shaffer KM, Hou YJ, Munt JE, Adams LE, Wykoff JA, Morowitz JM, Dong S, Magness ST, Marzluff WF, Gonzalez LM, Ehre C, Baric RS. Proc Natl Acad Sci U S A. 2022 May 3;119(18):e2118126119. doi: 10.1073/pnas.2118126119. Epub 2022 Apr 27. PubMed |
| A genome-wide CRISPR-Cas9 knockout screen identifies essential and growth-restricting genes in human trophoblast stem cells. Dong C, Fu S, Karvas RM, Chew B, Fischer LA, Xing X, Harrison JK, Popli P, Kommagani R, Wang T, Zhang B, Theunissen TW. Nat Commun. 2022 May 10;13(1):2548. doi: 10.1038/s41467-022-30207-9. PubMed |
| Inwardly rectifying potassium channels mediate polymyxin-induced nephrotoxicity. Lu J, Azad MAK, Moreau JLM, Zhu Y, Jiang X, Tonta M, Lam R, Wickremasinghe H, Zhao J, Wang J, Coleman HA, Formosa LE, Velkov T, Parkington HC, Combes AN, Rosenbluh J, Li J. Cell Mol Life Sci. 2022 May 15;79(6):296. doi: 10.1007/s00018-022-04316-z. PubMed |
| Chromatin Rewiring by Mismatch Repair Protein MSH2 Alters Cell Adhesion Pathways and Sensitivity to BET Inhibition in Gastric Cancer. Nargund AM, Xu C, Mandoli A, Okabe A, Chen GB, Huang KK, Sheng T, Yao X, Teo JMN, Sundar R, Kok YJ, See YX, Xing M, Li Z, Yong CH, Anand A, A I ZF, Poon LF, Ng MSW, Koh JYP, Ooi WF, Tay ST, Ong X, Tan ALK, Grabsch HI, Fullwood MJ, Teh TB, Bi X, Kaneda A, Li S, Tan P. Cancer Res. 2022 Jul 18;82(14):2538-2551. doi: 10.1158/0008-5472.CAN-21-2072. PubMed |
| Mutations in ALK signaling pathways conferring resistance to ALK inhibitor treatment lead to collateral vulnerabilities in neuroblastoma cells. Berlak M, Tucker E, Dorel M, Winkler A, McGearey A, Rodriguez-Fos E, da Costa BM, Barker K, Fyle E, Calton E, Eising S, Ober K, Hughes D, Koutroumanidou E, Carter P, Stankunaite R, Proszek P, Jain N, Rosswog C, Dorado-Garcia H, Molenaar JJ, Hubank M, Barone G, Anderson J, Lang P, Deubzer HE, Kunkele A, Fischer M, Eggert A, Kloft C, Henssen AG, Boettcher M, Hertwig F, Bluthgen N, Chesler L, Schulte JH. Mol Cancer. 2022 Jun 10;21(1):126. doi: 10.1186/s12943-022-01583-z. PubMed |
| The TIP60-ATM axis regulates replication fork stability in BRCA-deficient cells. Schleicher EM, Dhoonmoon A, Jackson LM, Khatib JB, Nicolae CM, Moldovan GL. Oncogenesis. 2022 Jun 18;11(1):33. doi: 10.1038/s41389-022-00410-w. PubMed |
| Reversal of viral and epigenetic HLA class I repression in Merkel cell carcinoma. Lee PC, Klaeger S, Le PM, Korthauer K, Cheng J, Ananthapadmanabhan V, Frost TC, Stevens JD, Wong AY, Iorgulescu JB, Tarren AY, Chea VA, Carulli IP, Lemvigh CK, Pedersen CB, Gartin AK, Sarkizova S, Wright KT, Li LW, Nomburg J, Li S, Huang T, Liu X, Pomerance L, Doherty LM, Apffel AM, Wallace LJ, Rachimi S, Felt KD, Wolff JO, Witten E, Zhang W, Neuberg D, Lane WJ, Zhang G, Olsen LR, Thakuria M, Rodig SJ, Clauser KR, Starrett GJ, Doench JG, Buhrlage SJ, Carr SA, DeCaprio JA, Wu CJ, Keskin DB. J Clin Invest. 2022 Jul 1;132(13). pii: 151666. doi: 10.1172/JCI151666. PubMed |
| Genome-wide bidirectional CRISPR screens identify mucins as host factors modulating SARS-CoV-2 infection. Biering SB, Sarnik SA, Wang E, Zengel JR, Leist SR, Schafer A, Sathyan V, Hawkins P, Okuda K, Tau C, Jangid AR, Duffy CV, Wei J, Gilmore RC, Alfajaro MM, Strine MS, Nguyenla X, Van Dis E, Catamura C, Yamashiro LH, Belk JA, Begeman A, Stark JC, Shon DJ, Fox DM, Ezzatpour S, Huang E, Olegario N, Rustagi A, Volmer AS, Livraghi-Butrico A, Wehri E, Behringer RR, Cheon DJ, Schaletzky J, Aguilar HC, Puschnik AS, Button B, Pinsky BA, Blish CA, Baric RS, O'Neal WK, Bertozzi CR, Wilen CB, Boucher RC, Carette JE, Stanley SA, Harris E, Konermann S, Hsu PD. Nat Genet. 2022 Aug;54(8):1078-1089. doi: 10.1038/s41588-022-01131-x. Epub 2022 Jul 25. PubMed |
| Targeting OXPHOS de novo purine synthesis as the nexus of FLT3 inhibitor-mediated synergistic antileukemic actions. Zhang P, Brinton LT, Gharghabi M, Sher S, Williams K, Cannon M, Walker JS, Canfield D, Beaver L, Cempre CB, Phillips H, Chen X, Yan P, Lehman A, Scherle P, Wang M, Vaddi K, Baiocchi R, Wang R, Sampath D, Alinari L, Blachly JS, Lapalombella R. Sci Adv. 2022 Sep 16;8(37):eabp9005. doi: 10.1126/sciadv.abp9005. Epub 2022 Sep 16. PubMed |
| NUDT21 limits CD19 levels through alternative mRNA polyadenylation in B cell acute lymphoblastic leukemia. Witkowski MT, Lee S, Wang E, Lee AK, Talbot A, Ma C, Tsopoulidis N, Brumbaugh J, Zhao Y, Roberts KG, Hogg SJ, Nomikou S, Ghebrechristos YE, Thandapani P, Mullighan CG, Hochedlinger K, Chen W, Abdel-Wahab O, Eyquem J, Aifantis I. Nat Immunol. 2022 Oct;23(10):1424-1432. doi: 10.1038/s41590-022-01314-y. Epub 2022 Sep 22. PubMed |
| TP53 mutations and RNA-binding protein MUSASHI-2 drive resistance to PRMT5-targeted therapy in B-cell lymphoma. Erazo T, Evans CM, Zakheim D, Chu KL, Refermat AY, Asgari Z, Yang X, Da Silva Ferreira M, Mehta S, Russo MV, Knezevic A, Zhang XP, Chen Z, Fennell M, Garippa R, Seshan V, de Stanchina E, Barbash O, Batlevi CL, Leslie CS, Melnick AM, Younes A, Kharas MG. Nat Commun. 2022 Sep 27;13(1):5676. doi: 10.1038/s41467-022-33137-8. PubMed |
| Complementary CRISPR genome-wide genetic screens in PARP10-knockout and overexpressing cells identify synthetic interactions for PARP10-mediated cellular survival. Khatib JB, Schleicher EM, Jackson LM, Dhoonmoon A, Moldovan GL, Nicolae CM. Oncotarget. 2022 Sep 28;13:1078-1091. doi: 10.18632/oncotarget.28277. eCollection 2022. PubMed |
| NOTCH Signaling Limits the Response of Low-Grade Serous Ovarian Cancers to MEK Inhibition. Llaurado Fernandez M, Hijmans EM, Gennissen AMC, Wong NKY, Li S, Wisman GBA, Hamilton A, Hoenisch J, Dawson A, Lee CH, Bittner M, Kim H, DiMattia GE, Lok CAR, Lieftink C, Beijersbergen RL, de Jong S, Carey MS, Bernards R, Berns K. Mol Cancer Ther. 2022 Dec 2;21(12):1862-1874. doi: 10.1158/1535-7163.MCT-22-0004. PubMed |
| Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism. Fu Y, Xiong S. PLoS Pathog. 2023 Jan 12;19(1):e1011090. doi: 10.1371/journal.ppat.1011090. eCollection 2023 Jan. PubMed |
| Thioparib inhibits homologous recombination repair, activates the type I IFN response, and overcomes olaparib resistance. Wang LM, Wang P, Chen XM, Yang H, Song SS, Song Z, Jia L, Chen HD, Bao XB, Guo N, Huan XJ, Xi Y, Shen YY, Yang XY, Su Y, Sun YM, Gao YL, Chen Y, Ding J, Lang JY, Miao ZH, Zhang A, He JX. EMBO Mol Med. 2023 Mar 8;15(3):e16235. doi: 10.15252/emmm.202216235. Epub 2023 Jan 18. PubMed |
| CRISPR screening reveals a dependency on ribosome recycling for efficient SARS-CoV-2 programmed ribosomal frameshifting and viral replication. Rehfeld F, Eitson JL, Ohlson MB, Chang TC, Schoggins JW, Mendell JT. Cell Rep. 2023 Jan 30;42(2):112076. doi: 10.1016/j.celrep.2023.112076. PubMed |
| Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis. Nassour J, Aguiar LG, Correia A, Schmidt TT, Mainz L, Przetocka S, Haggblom C, Tadepalle N, Williams A, Shokhirev MN, Akincilar SC, Tergaonkar V, Shadel GS, Karlseder J. Nature. 2023 Feb;614(7949):767-773. doi: 10.1038/s41586-023-05710-8. Epub 2023 Feb 8. PubMed |
| VMP1 prevents Ca2+ overload in endoplasmic reticulum and maintains naive T cell survival. Liu Y, Ma Y, Xu J, Zhang G, Zhao X, He Z, Wang L, Yin N, Peng M. J Exp Med. 2023 Jun 5;220(6):e20221068. doi: 10.1084/jem.20221068. Epub 2023 Mar 27. PubMed |
| Myeloid-associated differentiation marker is an essential host factor for human parechovirus PeV-A3 entry. Watanabe K, Oka T, Takagi H, Anisimov S, Yamashita SI, Katsuragi Y, Takahashi M, Higuchi M, Kanki T, Saitoh A, Fujii M. Nat Commun. 2023 Mar 31;14(1):1817. doi: 10.1038/s41467-023-37399-8. PubMed |
| The APE2 nuclease is essential for DNA double-strand break repair by microhomology-mediated end joining. Fleury H, MacEachern MK, Stiefel CM, Anand R, Sempeck C, Nebenfuehr B, Maurer-Alcala K, Ball K, Proctor B 3rd, Belan O, Taylor E, Ortega R, Dodd B, Weatherly L, Dansoko D, Leung JW, Boulton SJ, Arnoult N. Mol Cell. 2023 May 4;83(9):1429-1445.e8. doi: 10.1016/j.molcel.2023.03.017. Epub 2023 Apr 11. PubMed |
| A genome-wide CRISPR screen maps endogenous regulators of PPARG gene expression in bladder cancer. Tortora D, Roberts ME, Kumar G, Kotapalli SS, Ritch E, Scurll JM, McConeghy B, Sinha S, Wyatt AW, Black PC, Daugaard M. iScience. 2023 Mar 30;26(5):106525. doi: 10.1016/j.isci.2023.106525. eCollection 2023 May 19. PubMed |
| Functional CRISPR screens in T cells reveal new opportunities for cancer immunotherapies. Xiang M, Li H, Zhan Y, Ma D, Gao Q, Fang Y. Mol Cancer. 2024 Apr 5;23(1):73. doi: 10.1186/s12943-024-01987-z. PubMed |
| NSD2 drives t(4;14) myeloma cell dependence on adenylate kinase 2 by diverting one-carbon metabolism to the epigenome. Sobh A, Encinas E, Patel A, Surapaneni G, Bonilla E, Kaestner C, Poullard J, Clerio M, Vasan K, Freeman T, Lv D, Dupere-Richer D, Riva A, Barwick BG, Zhou D, Boise LH, Mitsiades CS, Kim B, Bennett RL, Chandel NS, Licht JD. Blood. 2024 Jul 18;144(3):283-295. doi: 10.1182/blood.2023022859. PubMed |
| An in vitro CRISPR screen of cell-free DNA identifies apoptosis as the primary mediator of cell-free DNA release. Davidson BA, Miranda AX, Reed SC, Bergman RE, Kemp JDJ, Reddy AP, Pantone MV, Fox EK, Dorand RD, Hurley PJ, Croessmann S, Park BH. Commun Biol. 2024 Apr 10;7(1):441. doi: 10.1038/s42003-024-06129-1. PubMed |
| CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single stranded DNA gaps. Pale LM, Khatib JB, Nicolae CM, Moldovan GL. bioRxiv [Preprint]. 2024 Apr 20:2024.04.17.589988. doi: 10.1101/2024.04.17.589988. PubMed |
| CRISPR-Cas and CRISPR-based screening system for precise gene editing and targeted cancer therapy. Qin M, Deng C, Wen L, Luo G, Meng Y. J Transl Med. 2024 May 30;22(1):516. doi: 10.1186/s12967-024-05235-2. PubMed |
| SMAD4 depletion contributes to endocrine resistance by integrating ER and ERBB signaling in HR + HER2- breast cancer. Li K, Shu D, Li H, Lan A, Zhang W, Tan Z, Huang M, Tomasi ML, Jin A, Yu H, Shen M, Liu S. Cell Death Dis. 2024 Jun 24;15(6):444. doi: 10.1038/s41419-024-06838-9. PubMed |
| Evolution of STAT2 resistance to flavivirus NS5 occurred multiple times despite genetic constraints. Veit EC, Salim MS, Jung MJ, Richardson RB, Boys IN, Quinlan M, Barrall EA, Bednarski E, Hamilton RE, Kikawa C, Elde NC, Garcia-Sastre A, Evans MJ. Nat Commun. 2024 Jun 26;15(1):5426. doi: 10.1038/s41467-024-49758-0. PubMed |
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