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pPB-CAG-mNanog-mut-pgk-hph
(Plasmid #74914)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74914 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nanog
  • Species
    M. musculus (mouse)
  • Mutation
    K43T of Nanog protein and Y42E of Nanog homeodomain (position 137 of Nanog protein)
  • Entrez Gene
    Nanog (a.k.a. 2410002E02Rik, EN, ENK, ecat, ecat4)
  • Promoter CAG

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-CAG-mNanog-mut-pgk-hph was a gift from Jose Silva (Addgene plasmid # 74914 ; http://n2t.net/addgene:74914 ; RRID:Addgene_74914)
  • For your References section:

    Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, Pain B, Castro LF, Silva JC. Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775. 10.1242/dev.068775 PubMed 22028025