Purpose(Empty Backbone) Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||75288||Plasmid sent as bacteria in agar stab||1||$65 *|
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Backbone manufacturerNew England Biolabs
- Backbone size (bp) 6645
Modifications to backbonemalE A313V
Vector typeBacterial Expression
- Promoter tac
/ Fusion Protein
- maltose-binding protein (N terminal on backbone)
Growth in Bacteria
Growth instructionsAny E. coli strain can be used for expression of fusion proteins; NEBExpress, BL21, or BL21(DE3) are good first choices
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer GGTCGTCAGACTGTCGATGAAGCC
- 3′ sequencing primer CGCCAGGGTTTTCCCAGTCACGAC (Common Sequencing Primers)
Commercial use requires a license from New England Biolabs
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMAL-c4X was a gift from Paul Riggs (Addgene plasmid # 75288)
For your References section:Mutations in maltose-binding protein that alter affinity and solubility properties. Walker IH, Hsieh PC, Riggs PD. Appl Microbiol Biotechnol. 2010 Sep;88(1):187-97. doi: 10.1007/s00253-010-2696-y. Epub 2010 Jun 10. 10.1007/s00253-010-2696-y PubMed 20535468
Generated by Addgene from full sequence supplied by depositor.