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pEB1deltaC-mCherry
(Plasmid #80603)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80603 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EB1
  • Alt name
    MAPRE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    747
  • Mutation
    Truncated C-terminus; EB1 coding sequence ends at threonine 249
  • GenBank ID
    NM_012325
  • Entrez Gene
    MAPRE1 (a.k.a. EB1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer mCherry reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEB1deltaC-mCherry was a gift from Torsten Wittmann (Addgene plasmid # 80603)
  • For your References section:

    Doublecortin Is Excluded from Growing Microtubule Ends and Recognizes the GDP-Microtubule Lattice. Ettinger A, van Haren J, Ribeiro SA, Wittmann T. Curr Biol. 2016 Jun 20;26(12):1549-55. doi: 10.1016/j.cub.2016.04.020. Epub 2016 May 26. 10.1016/j.cub.2016.04.020 PubMed 27238282