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Addgene

pIRES-EGFP2-AXL-Y481F
(Plasmid #80838)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80838 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    IRES2-EGFP
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 5308
  • Total vector size (bp) 7927
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tyrosine-protein kinase receptor UFO
  • Alt name
    AXL
  • Alt name
    UFO
  • Alt name
    AXL oncogene
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2619
  • Mutation
    Y481F
  • GenBank ID
    AAH32229.1
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer CTGGTCGAGCTGGACGGCGACG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-EGFP2-AXL-Y481F was a gift from Aaron Meyer (Addgene plasmid # 80838)
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