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pAD-CMV-Caveolin1-CMV-GFP
(Plasmid #83272)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83272 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    CAV1
  • Alt name
    caveolin 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    CAV1 (a.k.a. BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21)
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer T7
  • 3′ sequencing primer SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    eGFP
  • Species
    jellyfish
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Gateway Cloning
  • 5′ sequencing primer LNCX (5'-AGCTCGTTTAGTGAACCGTCAGATC-3')
  • 3′ sequencing primer TK-pA-R2 (5'-GGTGGGGAAAAGGAAGAAAC-3')
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAD-CMV-Caveolin1-CMV-GFP was a gift from Andrew Brooks (Addgene plasmid # 83272 ; http://n2t.net/addgene:83272 ; RRID:Addgene_83272)
  • For your References section:

    Caveolin-1 is necessary for hepatic oxidative lipid metabolism: evidence for crosstalk between caveolin-1 and bile acid signaling. Fernandez-Rojo MA, Gongora M, Fitzsimmons RL, Martel N, Martin SD, Nixon SJ, Brooks AJ, Ikonomopoulou MP, Martin S, Lo HP, Myers SA, Restall C, Ferguson C, Pilch PF, McGee SL, Anderson RL, Waters MJ, Hancock JF, Grimmond SM, Muscat GE, Parton RG. Cell Rep. 2013 Jul 25;4(2):238-47. doi: 10.1016/j.celrep.2013.06.017. Epub 2013 Jul 11. 10.1016/j.celrep.2013.06.017 PubMed 23850288