pAD-CMV-Caveolin1-CMV-GFP
(Plasmid #83272)

• Purpose: Adenoviral expression of Caveolin1 with co-expression of GFP
• Depositing Lab: Andrew Brooks
• Publication: Fernandez-Rojo et al Cell Rep. 2013 Jul 25;4(2):238-47. doi: 10.1016/j.celrep.2013.06.017. Epub 2013 Jul 11.

Backbone

• Vector backbone: pAD/CMV/V5-DEST
• Backbone manufacturer: Invitrogen
• Vector type: Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: CAV1
• Alt name: caveolin 1
• Species: H. sapiens (human)
• Entrez Gene: CAV1 (a.k.a. BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21)
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')

Gene/Insert 2

• Gene/Insert name: eGFP
• Species: jellyfish
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: LNCX (5'-AGCTCGTTTAGTGAACCGTCAGATC-3')
• 3′ sequencing primer: TK-pA-R2 (5'-GGTGGGGAAAAGGAAGAAAC-3')

Resource Information

• Supplemental Documents:
  • pAD-CMV-Caveolin1-CMV-GFP-GenBank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry