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HOXA9 (murine) FLAG pRC/CMV
(Plasmid #8514)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8514 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRC/CMV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HOXA9
  • Alt name
    HOX1G
  • Alt name
    HOX1.7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1114
  • Mutation
    FLAG epitope tag replaces T7 tag in the pET backbone; ribosomal binding site, HIS tag and new FLAG from pET cloned into pRC/CMV
  • GenBank ID
    NM_010456
  • Entrez Gene
    Hoxa9 (a.k.a. D6a9, Hox-1.7)
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindII/blunt-Xba/blunt (destroyed during cloning)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Debra Wolgemuth
  • Terms and Licenses

Depositor Comments

this clone lacks most of the long 3'UTR from the longest Hoxa9 gene transcript

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXA9 (murine) FLAG pRC/CMV was a gift from Corey Largman (Addgene plasmid # 8514 ; http://n2t.net/addgene:8514 ; RRID:Addgene_8514)
  • For your References section:

    AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins. Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, Buchberg AM, Largman C. Mol Cell Biol 1997 Nov;17(11):6448-58. PubMed 9343407