HOXA9 (murine) FLAG pRC/CMV
(Plasmid #8514)

• Depositing Lab: Corey Largman
• Publication: Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Backbone

• Vector backbone: pRC/CMV
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HOXA9
• Alt name: HOX1G
• Alt name: HOX1.7
• Species: M. musculus (mouse)
• Insert Size (bp): 1114
• Mutation: FLAG epitope tag replaces T7 tag in the pET backbone; ribosomal binding site, HIS tag and new FLAG from pET cloned into pRC/CMV
• GenBank ID: NM_010456
• Entrez Gene: Hoxa9 (a.k.a. D6a9, Hox-1.7)
• Tags / Fusion Proteins:
  • His (N terminal on insert)
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindII/blunt-Xba/blunt (destroyed during cloning)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Debra Wolgemuth

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

this clone lacks most of the long 3'UTR from the longest Hoxa9 gene transcript