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pUC18-H1 RNAi
(Plasmid #87355)


Item Catalog # Description Quantity Price (USD)
Plasmid 87355 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 2864
  • Modifications to backbone
    MCS is BglII-XbaI-HinDIII. H1 RNA promoter placed 5' to MCS.
  • Vector type
    Mammalian Expression, RNAi ; shRNA expression vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Can be maintained in E. coli SURE or any other recombination deficient strain
  • Copy number
    High Copy


  • Gene/Insert name
    H1 RNA Promoter from U87MG glioma cells
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Promoter H1 RNA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13 reverse sequencing primer
  • 3′ sequencing primer M13 forward sequencing primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

H1 RNA promoter cloned from U87MG glioma (brain tumor) cells via RT-PCR. Vector suitable for shRNA studies, particularly for shRNA expression in glioma cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC18-H1 RNAi was a gift from Saroj Mathupala (Addgene plasmid # 87355 ; ; RRID:Addgene_87355)
  • For your References section:

    Silencing of monocarboxylate transporters via small interfering ribonucleic acid inhibits glycolysis and induces cell death in malignant glioma: an in vitro study. Mathupala SP, Parajuli P, Sloan AE. Neurosurgery. 2004 Dec;55(6):1410-9; discussion 1419. PubMed 15574223