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(Plasmid #87944)


Item Catalog # Description Quantity Price (USD)
Plasmid 87944 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 9109
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Zif268 DBD - hPR LBD - MSN2 AD
  • Alt name
  • Species
    H. sapiens (human), S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
  • Entrez Gene
    PGR (a.k.a. NR3C3, PR)
  • Promoter ADH1 (crippled)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PspOMI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer GTGGATTCGGCTTTGGGTA
  • 3′ sequencing primer CGCACTCACGTAAACACTTAATC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHES830 was a gift from Hana El-Samad (Addgene plasmid # 87944 ; ; RRID:Addgene_87944)
  • For your References section:

    Robust Synthetic Circuits for Two-Dimensional Control of Gene Expression in Yeast. Aranda-Diaz A, Mace K, Zuleta I, Harrigan P, El-Samad H. ACS Synth Biol. 2016 Dec 27. doi: 10.1021/acssynbio.6b00251. 10.1021/acssynbio.6b00251 PubMed 27930885