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Robust Synthetic Circuits for Two-Dimensional Control of Gene Expression in Yeast.
Aranda-Diaz A, Mace K, Zuleta I, Harrigan P, El-Samad H
ACS Synth Biol. 2016 Dec 27. doi: 10.1021/acssynbio.6b00251.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87941pHES839Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.
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87942pHES941Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.
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87943pHES795Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.
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87944pHES830Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.
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87945pHES873Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.
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87946pHES835Expresses YFP under control of synthetic promoter containing Zif268 binding sites.
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87947pHES834Expresses mKate2 under control of GAL1 promoter.
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89190pHES820Expresses YFP under control of GAL1 promoter.
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89191pHES840Expresses YFP under control of GAL1 promoter.
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89192pHES875Expresses mCherry under control of GAL1 promoter.
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89193pHES822Expresses YFP under control of synthetic promoter containing Zif268 binding sites.
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89194pHES841Expresses YFP under control of synthetic promoter containing Zif268 binding sites.
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89195pHES836Expresses mKate2 under control of synthetic promoter containing Zif268 binding sites.
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89196pHES874Expresses mCherry under control of synthetic promoter containing Zif268 binding sites.
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89197pHES854Expresses mKate2 under control of TDH3 constitutive promoter.
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89199pHES936Expresses DIG1 under control of GAL1 promoter
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89200pHES938Expresses MSG5 under control of GAL1 promoter
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89201pHES939Expresses GPA1 under control of GAL1 promoter
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89202pHES940Expresses STE4 under control of synthetic promoter containing Zif268 binding sites.
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90408pHES942Expresses SIC1 udner control of synthetic promoter containing Zif268 binding sites.
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90409pHES833Expresses YFP under control of GAL1 promoter
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