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Addgene

MCL-1-GLuc1
(Plasmid #88931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 88931 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1/Zeo(+)
  • Backbone size w/o insert (bp) 4400
  • Total vector size (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BCL2L3
  • Alt name
    MCL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1183
  • GenBank ID
    NM_021960
  • Promoter CMV
  • Tag / Fusion Protein
    • GLuc1 (C terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MCL-1-GLuc1 was a gift from Adi Kimchi (Addgene plasmid # 88931)

  • For your References section:

    Discovering protein-protein interactions within the programmed cell death network using a protein-fragment complementation screen. Gilad Y, Shiloh R, Ber Y, Bialik S, Kimchi A. Cell Rep. 2014 Aug 7;8(3):909-21. doi: 10.1016/j.celrep.2014.06.049. Epub 2014 Jul 24. 10.1016/j.celrep.2014.06.049 PubMed 25066129
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