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Addgene

Tyrosinase H-ras Val12
(Plasmid #8899)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8899 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript KS
  • Backbone size w/o insert (bp) 13000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ras val12
  • Alt name
    ras
  • Alt name
    H-ras
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Mutation
    G12V
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
  • Tag / Fusion Protein
    • Tyr enhancer & promoter (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    RasV12 was isolated from a fragment of pRIPI-cH-Ras, a gift of Shimon Efrat.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The construct is composed of the following elements from 5' to 3'. 1) 9.1kb tyrosinase enhancer and promoter, 2) 1.8kb rasVal12, 3) SV40 splice and polyadenylation sequences. The entire 11.8kb insert containing rasV12 and regulatory elements can be released with NotI. See author's map for more information. Please also cite: Kaestner et al. Genes Dev 1997; 11(12):1583-95.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tyrosinase H-ras Val12 was a gift from Lynda Chin (Addgene plasmid # 8899)
  • For your References section:

    Cooperative effects of INK4a and ras in melanoma susceptibility in vivo. Chin L, Pomerantz J, Polsky D, Jacobson M, Cohen C, Cordon-Cardo C, Horner JW, DePinho RA. Genes Dev. 1997 Nov 1. 11(21):2822-34. 10.1101/gad.11.21.2822 PubMed 9353252