Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

tet H-ras val12
(Plasmid #8900)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8900 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript KS
  • Backbone size w/o insert (bp) 3350
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ras val12
  • Alt name
    ras
  • Alt name
    H-ras
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • Mutation
    G12V
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
  • Tag / Fusion Protein
    • Tet-op promoter (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    RasV12 was isolated from a fragment of pRIPI-cH-Ras, a gift of Shimon Efrat.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The construct is composed of the following elements: 1) 350bp Tet-operators with CMV minimal promoter, 2) 1.8kb rasVal12, 3) SV40 splice and polyadenylation sequences. The entire 3050bp insert containing rasV12 and regulatory elements can be released with NotI. See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tet H-ras val12 was a gift from Lynda Chin (Addgene plasmid # 8900 ; http://n2t.net/addgene:8900 ; RRID:Addgene_8900)
  • For your References section:

    Essential role for oncogenic Ras in tumour maintenance. Chin L, Tam A, Pomerantz J, Wong M, Holash J, Bardeesy N, Shen Q, O'Hagan R, Pantginis J, Zhou H, Horner JW, Cordon-Cardo C, Yancopoulos GD, DePinho RA. Nature. 1999 Jul 29. 400(6743):468-72. 10.1038/22788 PubMed 10440378