tet H-ras val12
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8900||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS
- Backbone size w/o insert (bp) 3350
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameras val12
SpeciesH. sapiens (human)
Insert Size (bp)2700
Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
/ Fusion Protein
- Tet-op promoter (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byRasV12 was isolated from a fragment of pRIPI-cH-Ras, a gift of Shimon Efrat.
Terms and Licenses
- Not Available to Industry
The construct is composed of the following elements: 1) 350bp Tet-operators with CMV minimal promoter, 2) 1.8kb rasVal12, 3) SV40 splice and polyadenylation sequences. The entire 3050bp insert containing rasV12 and regulatory elements can be released with NotI. See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:tet H-ras val12 was a gift from Lynda Chin (Addgene plasmid # 8900 ; http://n2t.net/addgene:8900 ; RRID:Addgene_8900)
For your References section:Essential role for oncogenic Ras in tumour maintenance. Chin L, Tam A, Pomerantz J, Wong M, Holash J, Bardeesy N, Shen Q, O'Hagan R, Pantginis J, Zhou H, Horner JW, Cordon-Cardo C, Yancopoulos GD, DePinho RA. Nature. 1999 Jul 29. 400(6743):468-72. 10.1038/22788 PubMed 10440378
Map uploaded by the depositor.