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(Plasmid #90242)


Item Catalog # Description Quantity Price (USD)
Plasmid 90242 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
    Tet-pLKO-Puro (pLKO-Tet-On) Addgene #21915
  • Backbone manufacturer
    Dmitri Wiederschain
  • Backbone size w/o insert (bp) 8758
  • Total vector size (bp) 8815
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • gRNA/shRNA sequence
  • Species
    H. sapiens (human)
  • Entrez Gene
    HDAC1 (a.k.a. GON-10, HD1, KDAC1, RPD3, RPD3L1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer SHSEQ2 GGAAATCACCATAAACGTGA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO-Tet-On-HDAC1-shRNA1 was a gift from Roland Friedel (Addgene plasmid # 90242 ; ; RRID:Addgene_90242)