Toronto KnockOut version 3 library (TKOv3) Citations (42)
Originally described in: Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens.Hart T, Tong AHY, Chan K, Van Leeuwen J, Seetharaman A, Aregger M, Chandrashekhar M, Hustedt N, Seth S, Noonan A, Habsid A, Sizova O, Nedyalkova L, Climie R, Tworzyanski L, Lawson K, Sartori MA, Alibeh S, Tieu D, Masud S, Mero P, Weiss A, Brown KR, Usaj M, Billmann M, Rahman M, Constanzo M, Myers CL, Andrews BJ, Boone C, Durocher D, Moffat J G3 (Bethesda). 2017 Aug 7;7(8):2719-2727. doi: 10.1534/g3.117.041277. PubMed Journal
Articles Citing Toronto KnockOut version 3 library (TKOv3)
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| Genetic interaction mapping and exon-resolution functional genomics with a hybrid Cas9-Cas12a platform. Gonatopoulos-Pournatzis T, Aregger M, Brown KR, Farhangmehr S, Braunschweig U, Ward HN, Ha KCH, Weiss A, Billmann M, Durbic T, Myers CL, Blencowe BJ, Moffat J. Nat Biotechnol. 2020 May;38(5):638-648. doi: 10.1038/s41587-020-0437-z. Epub 2020 Mar 16. PubMed |
| Multiple-gene targeting and mismatch tolerance can confound analysis of genome-wide pooled CRISPR screens. Fortin JP, Tan J, Gascoigne KE, Haverty PM, Forrest WF, Costa MR, Martin SE. Genome Biol. 2019 Jan 25;20(1):21. doi: 10.1186/s13059-019-1621-7. PubMed |
| Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells. MacLeod G, Bozek DA, Rajakulendran N, Monteiro V, Ahmadi M, Steinhart Z, Kushida MM, Yu H, Coutinho FJ, Cavalli FMG, Restall I, Hao X, Hart T, Luchman HA, Weiss S, Dirks PB, Angers S. Cell Rep. 2019 Apr 16;27(3):971-986.e9. doi: 10.1016/j.celrep.2019.03.047. PubMed |
| Identifying chemogenetic interactions from CRISPR screens with drugZ. Colic M, Wang G, Zimmermann M, Mascall K, McLaughlin M, Bertolet L, Lenoir WF, Moffat J, Angers S, Durocher D, Hart T. Genome Med. 2019 Aug 22;11(1):52. doi: 10.1186/s13073-019-0665-3. PubMed |
| Pooled CRISPR-Based Genetic Screens in Mammalian Cells. Chan K, Tong AHY, Brown KR, Mero P, Moffat J. J Vis Exp. 2019 Sep 4;(151). doi: 10.3791/59780. PubMed |
| Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex. van de Weijer ML, Krshnan L, Liberatori S, Guerrero EN, Robson-Tull J, Hahn L, Lebbink RJ, Wiertz EJHJ, Fischer R, Ebner D, Carvalho P. Mol Cell. 2020 Sep 3;79(5):768-781.e7. doi: 10.1016/j.molcel.2020.07.009. Epub 2020 Jul 31. PubMed |
| Discovery of Zika virus host dependency factors in trophoblasts using CRISPR/Cas9 screening. Rother M, Dimmler C, Weege F, Mollenkopf HJ, Meyer TF, Naumann M. J Virol Methods. 2021 Apr;290:114085. doi: 10.1016/j.jviromet.2021.114085. Epub 2021 Feb 2. PubMed |
| Intermedilysin cytolytic activity depends on heparan sulfates and membrane composition. Drabavicius G, Daelemans D. PLoS Genet. 2021 Feb 12;17(2):e1009387. doi: 10.1371/journal.pgen.1009387. eCollection 2021 Feb. PubMed |
| Signal peptidase complex subunit 1 is an essential Zika virus host factor in placental trophoblasts. Rother M, Naumann M. Virus Res. 2021 Apr 15;296:198338. doi: 10.1016/j.virusres.2021.198338. Epub 2021 Feb 9. PubMed |
| Genome-scale chemogenomic CRISPR screens in human cells using the TKOv3 library. Olivieri M, Durocher D. STAR Protoc. 2021 Feb 5;2(1):100321. doi: 10.1016/j.xpro.2021.100321. eCollection 2021 Mar 19. PubMed |
| Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages. Navarro-Guerrero E, Tay C, Whalley JP, Cowley SA, Davies B, Knight JC, Ebner D. Sci Rep. 2021 Feb 19;11(1):4245. doi: 10.1038/s41598-021-82137-z. PubMed |
| Genome wide CRISPR screening reveals a role for sialylation in the tumorigenesis and chemoresistance of acute myeloid leukemia cells. Lee DH, Kang SH, Choi DS, Ko M, Choi E, Ahn H, Min H, Oh SJ, Lee MS, Park Y, Jin HS. Cancer Lett. 2021 Jul 10;510:37-47. doi: 10.1016/j.canlet.2021.04.006. Epub 2021 Apr 16. PubMed |
| A method for benchmarking genetic screens reveals a predominant mitochondrial bias. Rahman M, Billmann M, Costanzo M, Aregger M, Tong AHY, Chan K, Ward HN, Brown KR, Andrews BJ, Boone C, Moffat J, Myers CL. Mol Syst Biol. 2021 May;17(5):e10013. doi: 10.15252/msb.202010013. PubMed |
| A KMT2A-AFF1 gene regulatory network highlights the role of core transcription factors and reveals the regulatory logic of key downstream target genes. Harman JR, Thorne R, Jamilly M, Tapia M, Crump NT, Rice S, Beveridge R, Morrissey E, de Bruijn MFTR, Roberts I, Roy A, Fulga TA, Milne TA. Genome Res. 2021 Jun 4. pii: gr.268490.120. doi: 10.1101/gr.268490.120. PubMed |
| The bacterial toxin ExoU requires a host trafficking chaperone for transportation and to induce necrosis. Deruelle V, Bouillot S, Job V, Taillebourg E, Fauvarque MO, Attree I, Huber P. Nat Commun. 2021 Jun 29;12(1):4024. doi: 10.1038/s41467-021-24337-9. PubMed |
Loss of Integrin-linked kinase sensitizes breast cancer to SRC inhibitors.
Beetham H, Griffith BGC, Murina O, Loftus AE, Parry DA, Temps C, Culley J, Muir M, Unciti-Broceta A, Sims AH, Byron A, Brunton VG.
Cancer Res. 2021 Dec 17. pii: 0008-5472.CAN-21-0373. doi: 10.1158/0008-5472.CAN-21-0373.
PubMed
Associated Plasmids |
| GCN2 kinase activation by ATP-competitive kinase inhibitors. Tang CP, Clark O, Ferrarone JR, Campos C, Lalani AS, Chodera JD, Intlekofer AM, Elemento O, Mellinghoff IK. Nat Chem Biol. 2022 Feb;18(2):207-215. doi: 10.1038/s41589-021-00947-8. Epub 2021 Dec 23. PubMed |
| PKCbetaII phosphorylates ACSL4 to amplify lipid peroxidation to induce ferroptosis. Zhang HL, Hu BX, Li ZL, Du T, Shan JL, Ye ZP, Peng XD, Li X, Huang Y, Zhu XY, Chen YH, Feng GK, Yang D, Deng R, Zhu XF. Nat Cell Biol. 2022 Jan;24(1):88-98. doi: 10.1038/s41556-021-00818-3. Epub 2022 Jan 13. PubMed |
| Characterization of a small molecule inhibitor of disulfide reductases that induces oxidative stress and lethality in lung cancer cells. Johnson FD, Ferrarone J, Liu A, Brandstadter C, Munuganti R, Farnsworth DA, Lu D, Luu J, Sihota T, Jansen S, Nagelberg A, Shi R, Forcina GC, Zhang X, Cheng GSW, Spencer Miko SE, de Rappard-Yuswack G, Sorensen PH, Dixon SJ, Guha U, Becker K, Djaballah H, Somwar R, Varmus H, Morin GB, Lockwood WW. Cell Rep. 2022 Feb 8;38(6):110343. doi: 10.1016/j.celrep.2022.110343. PubMed |
| PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer. Akasu-Nagayoshi Y, Hayashi T, Kawabata A, Shimizu N, Yamada A, Yokota N, Nakato R, Shirahige K, Okamoto A, Akiyama T. Cancer Sci. 2022 Jun;113(6):2034-2043. doi: 10.1111/cas.15358. Epub 2022 Apr 24. PubMed |
| P2RY2-AKT activation is a therapeutically actionable consequence of XPO1 inhibition in acute myeloid leukemia. Lin KH, Rutter JC, Xie A, Killarney ST, Vaganay C, Benaksas C, Ling F, Sodaro G, Meslin PA, Bassil CF, Fenouille N, Hoj J, Washart R, Ang HX, Cerda-Smith C, Chaintreuil P, Jacquel A, Auberger P, Forget A, Itzykson R, Lu M, Lin J, Pierobon M, Sheng Z, Li X, Chilkoti A, Owzar K, Rizzieri DA, Pardee TS, Benajiba L, Petricoin E, Puissant A, Wood KC. Nat Cancer. 2022 Jul;3(7):837-851. doi: 10.1038/s43018-022-00394-x. Epub 2022 Jun 6. PubMed |
| CRISPR/Cas9 Screening for Identification of Genes Required for the Growth of Ovarian Clear Cell Carcinoma Cells. Kawabata A, Hayashi T, Akasu-Nagayoshi Y, Yamada A, Shimizu N, Yokota N, Nakato R, Shirahige K, Okamoto A, Akiyama T. Curr Issues Mol Biol. 2022 Apr 7;44(4):1587-1596. doi: 10.3390/cimb44040108. PubMed |
| Genome-wide in vivo screen of circulating tumor cells identifies SLIT2 as a regulator of metastasis. Xia F, Ma Y, Chen K, Duong B, Ahmed S, Atwal R, Philpott D, Ketela T, Pantea J, Lin S, Angers S, Kelley SO. Sci Adv. 2022 Sep 2;8(35):eabo7792. doi: 10.1126/sciadv.abo7792. Epub 2022 Sep 2. PubMed |
| Regulated degradation of the inner nuclear membrane protein SUN2 maintains nuclear envelope architecture and function. Krshnan L, Siu WS, Van de Weijer M, Hayward D, Guerrero EN, Gruneberg U, Carvalho P. Elife. 2022 Nov 1;11:e81573. doi: 10.7554/eLife.81573. PubMed |
| A genome-wide CRISPR screen identifies CALCOCO2 as a regulator of beta cell function influencing type 2 diabetes risk. Rottner AK, Ye Y, Navarro-Guerrero E, Rajesh V, Pollner A, Bevacqua RJ, Yang J, Spigelman AF, Baronio R, Bautista A, Thomsen SK, Lyon J, Nawaz S, Smith N, Wesolowska-Andersen A, Fox JEM, Sun H, Kim SK, Ebner D, MacDonald PE, Gloyn AL. Nat Genet. 2023 Jan;55(1):54-65. doi: 10.1038/s41588-022-01261-2. Epub 2022 Dec 21. PubMed |
| Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors. Chan K, Farias AG, Lee H, Guvenc F, Mero P, Brown KR, Ward H, Billmann M, Aulakh K, Astori A, Haider S, Marcon E, Braunschweig U, Pu S, Habsid A, Yan Tong AH, Christie-Holmes N, Budylowski P, Ghalami A, Mubareka S, Maguire F, Banerjee A, Mossman KL, Greenblatt J, Gray-Owen SD, Raught B, Blencowe BJ, Taipale M, Myers C, Moffat J. Heliyon. 2023 Jan;9(1):e12744. doi: 10.1016/j.heliyon.2022.e12744. Epub 2022 Dec 30. PubMed |
| A genome-wide CRISPR functional survey of the human phagocytosis molecular machinery. Essletzbichler P, Sedlyarov V, Frommelt F, Soulat D, Heinz LX, Stefanovic A, Neumayer B, Superti-Furga G. Life Sci Alliance. 2023 Feb 1;6(4):e202201715. doi: 10.26508/lsa.202201715. Print 2023 Apr. PubMed |
| A Virus-Packageable CRISPR System Identifies Host Dependency Factors Co-Opted by Multiple HIV-1 Strains. Montoya VR, Ready TM, Felton A, Fine SR, OhAinle M, Emerman M. mBio. 2023 Feb 28;14(1):e0000923. doi: 10.1128/mbio.00009-23. Epub 2023 Feb 6. PubMed |
| The ribosomal protein S6 kinase alpha-1 (RPS6KA1) induces resistance to venetoclax/azacitidine in acute myeloid leukemia. Weidenauer K, Schmidt C, Rohde C, Pauli C, Blank MF, Heid D, Waclawiczek A, Corbacioglu A, Gollner S, Lotze M, Vierbaum L, Renders S, Krijgsveld J, Raffel S, Sauer T, Trumpp A, Pabst C, Muller-Tidow C, Janssen M. Leukemia. 2023 Aug;37(8):1611-1625. doi: 10.1038/s41375-023-01951-8. Epub 2023 Jul 6. PubMed |
The SUMO-NIP45 pathway processes toxic DNA catenanes to prevent mitotic failure.
Hertz EPT, Vega IA, Kruse T, Wang Y, Hendriks IA, Bizard AH, Eugui-Anta A, Hay RT, Nielsen ML, Nilsson J, Hickson ID, Mailand N.
Nat Struct Mol Biol. 2023 Jul 20. doi: 10.1038/s41594-023-01045-0.
PubMed
Associated Plasmids |
| DePARylation is critical for S phase progression and cell survival. Nie L, Wang C, Huang M, Liu X, Feng X, Tang M, Li S, Hang Q, Teng H, Shen X, Ma L, Gan B, Chen J. bioRxiv [Preprint]. 2024 Feb 2:2023.07.31.551317. doi: 10.1101/2023.07.31.551317. PubMed |
| Cooperative regulation of coupled oncoprotein synthesis and stability in triple-negative breast cancer by EGFR and CDK12/13. Ang HX, Sutiman N, Deng XL, Liu A, Cerda-Smith CG, Hutchinson HM, Kim H, Bartelt LC, Chen Q, Barrera A, Lin J, Sheng Z, McDowell IC, Reddy TE, Nicchitta CV, Wood KC. Proc Natl Acad Sci U S A. 2023 Sep 19;120(38):e2221448120. doi: 10.1073/pnas.2221448120. Epub 2023 Sep 11. PubMed |
| LKB1 suppresses growth and promotes the internalization of EGFR through the PIKFYVE lipid kinase. Ferrarone JR, Thomas J, Unni AM, Zheng Y, Nagiec MJ, Gardner EE, Mashadova O, Li K, Koundouros N, Montalbano A, Mustafa M, Cantley LC, Blenis J, Sanjana NE, Varmus H. bioRxiv [Preprint]. 2023 Oct 20:2023.10.19.563158. doi: 10.1101/2023.10.19.563158. PubMed |
| Optimized protocol for CRISPR knockout of human iPSC-derived macrophages. Navarro-Guerrero E, Baronio R, Tay C, Knight JC, Ebner DV. STAR Protoc. 2024 Mar 15;5(1):102903. doi: 10.1016/j.xpro.2024.102903. Epub 2024 Feb 23. PubMed |
| The PRC2.1 Subcomplex Opposes G1 Progression through Regulation of CCND1 and CCND2. Longhurst AD, Wang K, Suresh HG, Ketavarapu M, Ward HN, Jones IR, Narayan V, Hundley FV, Hassan AZ, Boone C, Myers CL, Shen Y, Ramani V, Andrews BJ, Toczyski DP. bioRxiv [Preprint]. 2024 Mar 19:2024.03.18.585604. doi: 10.1101/2024.03.18.585604. PubMed |
| DePARylation is critical for S phase progression and cell survival. Nie L, Wang C, Huang M, Liu X, Feng X, Tang M, Li S, Hang Q, Teng H, Shen X, Ma L, Gan B, Chen J. Elife. 2024 Apr 5;12:RP89303. doi: 10.7554/eLife.89303. PubMed |
| Identifying human pre-mRNA cleavage and polyadenylation factors by genome-wide CRISPR screens using a dual fluorescence readthrough reporter. Ni Z, Ahmed N, Nabeel-Shah S, Guo X, Pu S, Song J, Marcon E, Burke GL, Tong AHY, Chan K, Ha KCH, Blencowe BJ, Moffat J, Greenblatt JF. Nucleic Acids Res. 2024 May 8;52(8):4483-4501. doi: 10.1093/nar/gkae240. PubMed |
The TSC22D, WNK, and NRBP gene families exhibit functional buffering and evolved with Metazoa for cell volume regulation.
Xiao YX, Lee SY, Aguilera-Uribe M, Samson R, Au A, Khanna Y, Liu Z, Cheng R, Aulakh K, Wei J, Farias AG, Reilly T, Birkadze S, Habsid A, Brown KR, Chan K, Mero P, Huang JQ, Billmann M, Rahman M, Myers C, Andrews BJ, Youn JY, Yip CM, Rotin D, Derry WB, Forman-Kay JD, Moses AM, Pritisanac I, Gingras AC, Moffat J.
Cell Rep. 2024 Jul 23;43(7):114417. doi: 10.1016/j.celrep.2024.114417. Epub 2024 Jul 8.
PubMed
Associated Plasmids |
| Netrin signaling mediates survival of dormant epithelial ovarian cancer cells. Perampalam P, MacDonald JI, Zakirova K, Passos DT, Wasif S, Ramos-Valdes Y, Hervieu M, Mehlen P, Rottapel R, Gibert B, Correa RJM, Shepherd TG, Dick FA. Elife. 2024 Jul 18;12:RP91766. doi: 10.7554/eLife.91766. PubMed |
| Genome-Wide CRISPR Screen Identifies KEAP1 Perturbation as a Vulnerability of ARID1A-Deficient Cells. Fournier LA, Kalantari F, Wells JP, Lee JS, Trigo-Gonzalez G, Moksa MM, Smith T, White J, Shanks A, Wang SL, Su E, Wang Y, Huntsman DG, Hirst M, Stirling PC. Cancers (Basel). 2024 Aug 24;16(17):2949. doi: 10.3390/cancers16172949. PubMed |
The efflux pump ABCC1/MRP1 constitutively restricts PROTAC sensitivity in cancer cells.
Wolf G, Craigon C, Teoh ST, Essletzbichler P, Onstein S, Cassidy D, Uijttewaal ECH, Dvorak V, Cao Y, Bensimon A, Elling U, Ciulli A, Superti-Furga G.
Cell Chem Biol. 2024 Dec 24:S2451-9456(24)00489-6. doi: 10.1016/j.chembiol.2024.11.009.
PubMed
Associated Plasmids |
| The PRC2.1 subcomplex opposes G1 progression through regulation of CCND1 and CCND2. Longhurst AD, Wang K, Suresh HG, Ketavarapu M, Ward HN, Jones IR, Narayan V, Hundley FV, Hassan AZ, Boone C, Myers CL, Shen Y, Ramani V, Andrews BJ, Toczyski DP. Elife. 2025 Feb 4;13:RP97577. doi: 10.7554/eLife.97577. PubMed |
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