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pBAD mPlum
(Plasmid #91773)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 91773 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    0.02% (weight/volume) of Arabinose is necessary for fluorescent protein production.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    monomeric Plum
  • Alt name
    mPlum
  • Species
    Discosoma sp. (mushroom coral)
  • Insert Size (bp)
    735
  • Promoter araBAD
  • Tag / Fusion Protein
    • no tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBAD Forward
  • 3′ sequencing primer unecessary
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

These plasmids are used in University Molecular Biology Laboratories and by High School students to purify fluorescent proteins and create fluorescent protein plate artwork.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD mPlum was a gift from Erik Rodriguez & Roger Tsien (Addgene plasmid # 91773 ; http://n2t.net/addgene:91773 ; RRID:Addgene_91773)
  • For your References section:

    Evolution of new nonantibody proteins via iterative somatic hypermutation. Wang L, Jackson WC, Steinbach PA, Tsien RY. Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16745-9. Epub 2004 Nov 19. 10.1073/pnas.0407752101 PubMed 15556995