A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.

Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
68283	SepOTSβ	Phosphoserine orthogonal translation system
68284	SepOTSγ	Phosphoserine orthogonal translation system
68285	SepOTSδ	Phosphoserine orthogonal translation system
68286	SepOTSε	Phosphoserine orthogonal translation system
68287	SepOTSζ	Phosphoserine orthogonal translation system
68288	SepOTSη	Phosphoserine orthogonal translation system
68289	SepOTSθ	Phosphoserine orthogonal translation system
68290	SepOTSι	Phosphoserine orthogonal translation system
68291	SepOTSκ	Phosphoserine orthogonal translation system
68292	SepOTSλ	Phosphoserine orthogonal translation system (preferred variant)
68294	SepOTSν	Phosphoserine orthogonal translation system
68295	GFP E17TAG	E17TAG GFP reporter
68296	GFP S2TAG	S2TAG GFP reporter
68297	GFP Q157TAG	Q157TAG GFP reporter
68298	GFP S2TAG/E17TAG	S2TAG/E17TAG GFP reporter
68299	GFP E17TAG/Q157TAG	E17/Q157 TAG GFP reporter
68300	MBP-MEK1	N-terminal MBP tagged human MEK1 gene for bacterial expression
68301	MBP-MEK1 S218TAG	N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression
68302	MBP-MEK1 S222TAG	N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression
68305	Beta lactamase S68TAG	Beta lactamase S68TAG gene
68306	Strain: C321.ΔA	Genomically recoded strain of E. coli (no genomic TAG)
69118	E17TAG GFP zeo resistance	For E17TAG GFP reporter expression in non-zeocin resistant strains
198598	SupD v2	Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.