Claudia Vickers Lab:
Vickers Lab plasmids
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83521 | pTCW010 | Aromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha2 gene |
| 83522 | pTCW002 | pheromone mediated destablised-GFP expression in yeast |
| 83523 | pTCW009 | Aromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha1 gene |
| 83524 | pTCW005 | pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene |
| 83525 | pTCW007 | pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoter |
| 83526 | pTCW008 | pheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoter |
| 83527 | pTCW012 | Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator |
| 83530 | pTCW015 | UBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoter |
| 83531 | pTCW018 | Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin |
| 83532 | pTCW022 | pheromone mediated expression of UBiC, ARO4, and TKL1 |
| 83535 | PHBA01 | Constitutive TEF1 promoter mediated expression of the UBiC gene |
| 83536 | PHBA02 | Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes |
| 83537 | PHBA03 | Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes |
| 83557 | pILGFPB6 | Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene. |
| 83558 | pILGFPQ3 | Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene. |
| 83559 | pILGFPQ4 | Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene. |
| 83561 | pILGFPJ1 | Express yEGFP-ERG9 ER signal peptide under the control of TEF1 promoter |
| 83562 | pILGFPU0 (R) | Express yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoter |
| 185862 | pJCKan | The CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable marker |
| 185863 | pJCHph | The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker |
| 185871 | pILGFP1DFB | A parental plasmid for in vivo gene amplification at RPL25 locus |
| 185872 | pILGFP3A5C | A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference) |
| 185873 | pILGFP3AE4 | A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy) |
| 185874 | pILGFP3AG4 | A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy) |
| 185875 | pILGFP3AA5 | A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy) |
| 185876 | pILGFP3AG4ARSd | A plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy) |
| 185877 | pILGFP4BG6 | A parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locus |
| 185878 | pILGFP5EG3 | A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (1 copy reference) |
| 185879 | pILGFP5EA4 | A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy) |
| 185880 | pILGFP5EC4 | A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy) |
| 185881 | pILGFP5EF3 | A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (13 copy) |
| 185882 | pINER2R | In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy). |
| 185883 | pINER3R | In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy). |
| 185884 | pINER4R | In vivo gene amplification (HamAmp) of nerolidol synthetic module locus (25 copy). |
| 185885 | pIT6EG7m | A backbone plasmid for in vivo gene amplification (HamAmp) of a monoterpene synthetic module (+ GPP synthase without a monoterpene synthase) at RPL25 locus (14 copy) |
| 185886 | pIT6EG7ml | In vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (14 copy). |
| 185887 | pIT6EG7mlh | In vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (23 copy). |
| 185889 | pILAC2 | In vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locus |
| 185890 | pILAC3 | In vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locus |
| 185891 | pIAeBlue | In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus |
| 185892 | pIEforRed | In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus |
| 185893 | pPAeBlueHPV16LR | 2-micron plasmid of AeBlue + HPV16 L1 expression module at RPL25 locus |
| 185894 | pIAeBlueHPV16LR | In vivo gene amplification (HamAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus |
| 185895 | pINER5 | In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy). |
| 185896 | pIT7A6 | In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy). |