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Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo.

Buckley CE, Moore RE, Reade A, Goldberg AR, Weiner OD, Clarke JD
Dev Cell. 2016 Jan 11;36(1):117-26. doi: 10.1016/j.devcel.2015.12.011. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
154910pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.
154911pCS2+-Y276H PHYB-CAAX1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.
154913pCS2+-PIF6-EGFP1-100 amino acids of Arabidopsis PIF6 (Gene ID: 825382), tagged with EGFP fluorescent protein. Mammalian codon optimised.
154914pCS2+-Pard3-EGFP-PIF6Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).

Antibodies from Article