Compartmentalized partnered replication for the directed evolution of genetic parts and circuits.
Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD
Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. Epub 2017 Nov 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102789 | pRST11B-AS3_4 | Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA |
| 102790 | pQE 80L Kan T7WT | Expresses T7 RNA polymerase |
| 102791 | pACYC-GFPM2 | Expresses GFP under WT T7 promoter |
| 102792 | pACYC.GFP1Amb | Expresses GFP with a premature stop codon |
| 102793 | pACYC-Taq | Expresses Taq DNA polymerase under WT T7 promoter |
| 102794 | pACYC.Taq.1Amb | Expresses Taq DNA polymerases with a premature stop codon |