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Systemic AAV vectors for widespread and targeted gene delivery in rodents.

Challis RC, Ravindra Kumar S, Chan KY, Challis C, Beadle K, Jang MJ, Kim HM, Rajendran PS, Tompkins JD, Shivkumar K, Deverman BE, Gradinaru V
Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
104052pAAV-CAG-DIO-EYFPAn AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter
104053pAAV-GFAP-2xNLS-mTurquoise2An AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoter
104054pAAV-MBP-2xNLS-tdTomatoAn AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter
104055pAAV-CAG-eYFPAn AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter
104056pAAV-TRE-eYFPAn AAV genome with tet-inducible expression of the fluorescent protein eYFP
104057pAAV-TRE-DIO-eYFP-fAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence
104058pAAV-CAG-DIO-mRuby2AAV expression of Cre-dependent reporter mRuby2
104059pAAV-CAG-DIO-mTurquoise2AAV expression of Cre-dependent reporter mTurquoise2
104060pAAV-hSyn1-tdTomatofAlso known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing
104061pAAV-CAG-NLS-GFPAAV expression of nuclear localized GFP with SV40 NLS
117380pAAV-CAG-eYFP-3x-miR204-5p-TSAn AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes
117381pAAV-CAG-eYFP-3x-miR708-5p-TSAn AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons
117382pAAV-hSyn1-eYFPAn AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter
117383pAAV-TRE-DIO-eYFPAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP
117384pAAV-CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TSAn AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively
118025pAAV-hSyn1-2xNLS-mTurquoise2An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter

Antibodies from Article