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Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids.
Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington AD, Moran NA, Barrick JE
ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
110610pBTK509BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter
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110611pBTK510BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter
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110612pBTK563BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoter
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110613pBTK564BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter
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110614pBTK569BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter
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110615pBTK570BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter
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110616pBTK503BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter
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110617pBTK550dBTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase
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110618pBTK552BTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI.
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110619pBTK619BTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoter
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110620pBTK620BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter
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110621pBTK621BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter
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