Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids.
Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington AD, Moran NA, Barrick JE
ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 10.1021/acssynbio.7b00399. Epub 2018 Apr 13. PubMed Article
Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington AD, Moran NA, Barrick JE
ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 10.1021/acssynbio.7b00399. Epub 2018 Apr 13. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
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| 110610 | pBTK509 | BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter |
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| 110611 | pBTK510 | BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter |
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| 110612 | pBTK563 | BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoter |
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| 110613 | pBTK564 | BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter |
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| 110614 | pBTK569 | BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter |
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| 110615 | pBTK570 | BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter |
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| 110616 | pBTK503 | BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter |
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| 110617 | pBTK550d | BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase |
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| 110618 | pBTK552 | BTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI. |
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| 110619 | pBTK619 | BTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoter |
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| 110620 | pBTK620 | BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter |
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| 110621 | pBTK621 | BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter |
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