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Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation.
Weith M, Seiler J, van den Boom J, Kracht M, Hulsmann J, Primorac I, Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, Meyer H
Mol Cell. 2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 10.1016/j.molcel.2018.09.020.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113500pGEX-6P-1 p37Bacterial expression of GST-tagged p37
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113501pGEX-6P-1 p37deltaSEPBacterial expression of GST-tagged p37 lacking the SEP domain
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113502pGEX-6P-1 p37delta2-81Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81
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113503pGEX-6P-1 p37 SHPmutBacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box
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113504pGEX-6P-1 p37 deltaUBXBacterial expression of GST-tagged p37 lacking the UBX domain
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113505pGEX-4T-1 UBXN2ABacterial expression of GST-tagged UBXN2A
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113506pcDNA5FRT/TO PP1gamma-2*StrepExpresses dual Strep-tagged PP1gamma in mammalian cells
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113510pFL His-p97Expression of His-p97 in insect cells (Baculovirus system)
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113511pFL His-SDS22 / PP1gammaExpression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)
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113512pFL His-SDS22Expression of His-SDS22 in insect cells (Baculovirus system)
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113513pFL His-I3Expression of His-I3 in insect cells (Baculovirus system)
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113514pFL I3Expression of I3 in insect cells (Baculovirus system)
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113515pFL His-I3 V41A / W43AExpression of His-I3 V41A / W43 A in insect cells (Baculovirus system)
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113516pFL His-mEos3.2-I3Expression of His-Eos-I3 in insect cells (Baculovirus system)
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117107pET41b+_Ufd1-HisProduction of recombinant Ufd1-His in E.coli
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117108pET41b+_Npl4Production of recombinant Npl4 in E.coli
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