Directed evolution improves the catalytic efficiency of TEV protease.
Sanchez MI, Ting AY
Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 10.1038/s41592-019-0665-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135452 | Gal:mCherry-CRY2-uTEV1Δ(220-242) | Yeast expression of uTEV1Δ fused to CRY2 and mCherry |
| 135453 | Gal:mCherry-uTEV3 | Yeast expression of uTEV3 fused to mCherry |
| 135454 | MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5 | Bacterial expression of TEVΔ protease |
| 135455 | MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5 | Bacterial expression of uTEV1Δ protease |
| 135456 | MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5 | Bacterial expression of uTEV2Δ protease |
| 135457 | UAS-mCherry | Mammalian mCherry reporter to read out FLARE and SPARK activation |
| 135458 | CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5 | Mammalian expression of SPARK TF component |
| 135459 | CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242) | Mammalian expression of SPARK protease component |
| 135460 | CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4 | Mammalian expression of uFLARE TF component |
| 135461 | CMV:eGFP-CaM-uTEV1Δ(220-242) | Mammalian expression of uFLARE protease component |
| 135462 | Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTA | Neuronal expression of uFLARE TF component |
| 135463 | Syn:eGFP-CaM-uTEV1Δ(220-242) | Neuronal expression of uFLARE protease component |
| 135464 | His6-MBP-uTEV3 | Bacterial expression of uTEV3 (full-length) protease |
| 135465 | TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC | Membrane-anchored TF used for yeast directed evolution of TEV protease |