A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair.

Nishimura K, Yamada R, Hagihara S, Iwasaki R, Uchida N, Kamura T, Takahashi K, Torii KU, Fukagawa T
Nucleic Acids Res. 2020 Oct 9;48(18):e108. doi: 10.1093/nar/gkaa748. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
140601	pAID1.2-CMV-NmCherry-mAID	Expression of OsTIR1 and mCherry-mAID protein in DT40 cells under the control of CMV promoter
140602	pAID1.2-CMV-CmCherry-mAID	Expression of OsTIR1 and mAID-mCherry protein in DT40 cells under the control of CMV promoter
140603	pAID1.2-EF1a-NmCherry-mAID	Expression of OsTIR1 and mCherry-mAID protein in mammalian cells under the control of EF1a promoter
140604	pAID1.2-EF1a-CmCherry-mAID	Expression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoter
140605	pAID1.2-CMV-NGFP-mAID	Expression of OsTIR1 and GFP-mAID protein in DT40 cells under the control of CMV promoter
140606	pAID1.2-CMV-CGFP-mAID	Expression of OsTIR1 and mAID-GFP protein in DT40 cells under the control of CMV promoter
140607	pAID1.2-EF1a-NGFP-mAID	Expression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoter
140608	pAID1.2-EF1a-CGFP-mAID	Expression of OsTIR1 and mAID-GFP protein in mammalian cells under the control of EF1a promoter
140609	pAID-CMV linearizing in pX330	Crispr/Cas9 plasmid for linearization of pAID-CMV
140610	pAID-EF1a linearizing in pX330	Crispr/Cas9 plasmid for linearization of pAID-EF1a
140615	pAIDFA-EF1a-NmScarlet-mAID	Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter
140616	pAIDFA-CMV-NmScarlet-mAID	Expression of OsTIR1F74A and mScarlet-mAID protein in DT40 cells under the control of CMV promoter
140617	pAID1.2-EF1a-NmScarlet-mAID	Expression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter
140618	pAID1.2-CMV-NmScarlet-mAID	Expression of OsTIR1 and mScarlet-mAID protein in DT40 cells under the control of CMV promoter