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The Scaffold Protein Axin Promotes Signaling Specificity within the Wnt Pathway by Suppressing Competing Kinase Reactions.

Gavagan M, Fagnan E, Speltz EB, Zalatan JG
Cell Syst. 2020 Jun 24;10(6):515-525.e5. doi: 10.1016/j.cels.2020.05.002. Epub 2020 Jun 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
154010pES001 MBP-GSK3β-HA-HisExpresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli
154011pEF073 MBP-Axin-HisExpresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli
154012pMG023 MBP-Axin 384-518-His (miniAxin)Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli
154053pMG035 MBP-Axin∆BCD-HisExpresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli
154070pEF019 MBP-β-catenin-HisExpresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli
154072pMG051 MBP-β-catenin-His, GST-CK1αCo-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His
154074pMG046 pETARA-CK1α-HisExpresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli
154075pEF086 MBP-CREB 127-135-HisExpresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli
154076pMG059 MBP-CREB-HisExpresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli
154077pMG058 MBP-GS 634-666-HisExpresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

Antibodies from Article