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Simultaneous quantification of mRNA and protein in single cells reveals post-transcriptional effects of genetic variation.

Brion C, Lutz SM, Albert FW
Elife. 2020 Nov 16;9. pii: 60645. doi: 10.7554/eLife.60645. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
157656Plas-gRNA-LEU2GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence
157658Plas-CRISPR_reporterCRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene
157659Plas-gRNA-Z3EVGFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.

Antibodies from Article