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Addgene
David Tollervey Lab: Tollervey SARS-CoV-2 plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose
157677pcDNA5-FRT-TO-N-Flag-8HisN-terminal tagging vector
157678pcDNA5-FRT-TO-C-8His-FlagC-terminal tagging vector
157679pcDNA5-FRT-TO-Nsp1mammalian expression of untagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter
157680pcDNA5-FRT-TO-FH-Nsp1mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter
157681pcDNA5-FRT-TO-Nsp1-HFmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter
157682pcDNA5-FRT-TO-Nsp2mammalian expression of untagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter
157683pcDNA5-FRT-TO-FH-Nsp2mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter
157684pcDNA5-FRT-TO-Nsp2-HFmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter
157685pcDNA5-FRT-TO-Nsp5mammalian expression of untagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter
157686pcDNA5-FRT-TO-FH-Nsp5mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter
157687pcDNA5-FRT-TO-Nsp5-HFmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter
157688pcDNA5-FRT-TO-Nsp7mammalian expression of untagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter
157689pcDNA5-FRT-TO-FH-Nsp7mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter
157690pcDNA5-FRT-TO-Nsp7-HFmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter
157691pcDNA-FRT-TO-Nsp7-Nsp8_GC3optmammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
157692pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3optmammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
157693pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3optmammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
157694pcDNA5-FRT-TO-Nsp8mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
157695pcDNA5-FRT-TO-FH-Nsp8mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
157696pcDNA5-FRT-TO-Nsp8-HFmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
157697pcDNA5-FRT-TO-Nsp8_GC3optmammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
157698pcDNA5-FRT-TO-FH-Nsp8_GC3optmammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
157699pcDNA5-FRT-TO-Nsp8-HF_GC3optmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
157700pcDNA-FRT-TO-Nsp8-Nsp7_GC3optmammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
157701pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3optmammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
157702pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3optmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
157703pcDNA5-FRT-TO-Nsp9mammalian expression of untagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter
157704pcDNA5-FRT-TO-FH-Nsp9mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter
157705pcDNA5-FRT-TO-Nsp9-HFmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter
157706pcDNA5-FRT-TO-Nsp10mammalian expression of untagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter
157707pcDNA5-FRT-TO-FH-Nsp10mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter
157708pcDNA5-FRT-TO-Nsp10-HFmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter
157709pcDNA5-FRT-TO-Nsp12mammalian expression of untagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter
157710pcDNA5-FRT-TO-FH-Nsp12mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter
157711pcDNA5-FRT-TO-Nsp12-HFmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter
157712pcDNA5-FRT-TO-Nsp13mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
157713pcDNA5-FRT-TO-FH-Nsp13mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
157714pcDNA5-FRT-TO-Nsp13_GC3optmammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
157715pcDNA5-FRT-TO-FH-Nsp13_GC3optmammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
157716pcDNA5-FRT-TO-Nsp13-HF_GC3optmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
157717pcDNA5-FRT-TO-Nsp14mammalian expression of untagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter
157718pcDNA5-FRT-TO-FH-Nsp14mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter
157719pcDNA5-FRT-TO-Nsp14-HFmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter
157720pcDNA5-FRT-TO-Nsp15mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter
157721pcDNA5-FRT-TO-FH-Nsp15mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter
157722pcDNA5-FRT-TO-Nsp15-HFmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter
157723pcDNA5-FRT-TO-Nsp16mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter
157724pcDNA5-FRT-TO-FH-Nsp16mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter
157725pcDNA5-FRT-TO-Nsp16-HFmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter
157726pcDNA5-FRT-TO-Orf6mammalian expression of untagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter
157727pcDNA5-FRT-TO-FH-Orf6mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter
157728pcDNA5-FRT-TO-Nprotmammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter
157729pcDNA5-FRT-TO-FH-Nprotmammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter
157730pcDNA-FRT-TO-Nprot_GC3optmammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter
157732pcDNA5-FRT-TO-Nprot-HF_GC3optmammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter
157733pcDNA5-FRT-TO-eGFPmammalian expression of untagged eGFP under control of a tetracycline-inducible promoter
157734pcDNA5-FRT-TO-FH-eGFPmammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoter
157735pcDNA5-FRT-TO-eGFP-HFmammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoter

Antibodies from Article