Kathleen Postle Lab:
Postle Lab Plasmids
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160815 | pKP1469 | Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD) |
| 167596 | pKP1963 | Express ExbB delta(195-204),ExbD |
| 167597 | pKP1964 | Express ExbB delta(205-214),ExbD |
| 167598 | pKP1965 | Express ExbB delta(215-224),ExbD |
| 167599 | pKP1940 | Express ExbB delta(225-234),ExbD |
| 167600 | pKP1912 | Express ExbB delta(235-244),ExbD |
| 167601 | pKP2008 | Express ExbB (205-214 Ala),ExbD |
| 167602 | pKP2009 | Express ExbB (215-224 Ala),ExbD |
| 167603 | pKP2010 | Express ExbB (235-244 Ala),ExbD |
| 167604 | pKP2019 | Express ExbB (195-204 Ala),ExbD |
| 167605 | pKP2025 | Express ExbB (225-234 Ala),ExbD |
| 167606 | pKP2085 | Express ColicinB-V5-TEV-T7-mature MBP |
| 167635 | pKP2185 | Express FepA D12C, I14P . Please note that mutation numbers are not based on start codon of insert. |
| 167637 | pKP2186 | Express FepA T13C, I14P . Please note that mutation numbers are not based on start codon of insert. |
| 167640 | pKP2187 | Express FepA V15C, I14P . Please note that mutation numbers are not based on start codon of insert. |
| 167642 | pKP2188 | Express FepA V16C, I14P . Please note that mutation numbers are not based on start codon of insert. |
| 167644 | pKP2189 | Express FepA T17C, I14P . Please note that mutation numbers are not based on start codon of insert. |
| 167649 | pKP1392 | Express ExbD T42C protein |
| 167650 | pKP2040 | Express His6-ExbB S80A, S82A, S84A |
| 167651 | pKP2032 | Express His6-ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A |
| 167653 | pKP968 | Express colicinB-His6, D17C, M261C |
| 167654 | pKP996 | Express colicinB-His6, T18C, M261C |
| 167655 | pKP995 | Express colicinB-His6, M19C, M261C |
| 167656 | pKP997 | Express colicinB-His6, V20C, M261C |
| 167658 | pKP864 | Express colicinB-His6 |
| 167660 | pKP2264 | Express colicinB-His6, A121C, Y254C |