Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking.
Sun X, Tie HC, Chen B, Lu L
J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476. PubMed Article
Sun X, Tie HC, Chen B, Lu L
J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162489 | GFP-Tac | Expresses Tac (IL2 recptor alpha subunit) with GFP tag in mammalian cells |
| 162490 | GFP-Tac-STC | Expresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells |
| 162491 | GFP-Tac-STC(5A) | Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells |
| 162492 | GFP-Tac-TC | Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in mammalian cells |
| 162493 | GFP-Tac(5A) | Expresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells |
| 162494 | Tac-GFP | Expresses Tac (IL2 recptor alpha subunit) with C-terminally tagged GFP in mammalian cells |
| 162495 | Tac(5A)-GFP | Expresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells |
| 162496 | GFP(N-glyc)-Tac-TC | Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag. |
| 162497 | GFP-Cstem-Tac(5A) | Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A). |
| 162498 | GFP-CD8a(7A) | Expresses GFP tagged CD8a mutant in mammalian cells. The seven O-glycosylation sites in its stem region are mutated. |
| 162499 | GFP-Cstem(7A)-Tac(5A) | Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A). |
| 162500 | ST-GFP | Expresses ST6GAL1 with a GFP tag in its C-terminus in mammalian cells |
| 162501 | ST-Tstem-GFP | Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST. |
| 162502 | ST-Tstem(5A)-GFP | Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST. |
| 162503 | ST-2xTstem-GFP | Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP. |
| 162504 | ST-2xTstem(5A)-GFP | Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP. |
| 162505 | SBP-GFP-Tac | Expresses GFP tagged Tac in a RUSH vector in mammalian cells |
| 162506 | SBP-GFP-Tac-TC | Expresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cells |