High-throughput cloning and expression of integral membrane proteins in Escherichia coli.

Bruni R, Kloss B
Curr Protoc Protein Sci. 2013 Nov 5;74:29.6.1-29.6.34. doi: 10.1002/0471140864.ps2906s74. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
165477	pC23-LIC-His-FLAG	pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His
165478	pN23-LIC-His-FLAG	pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His
165479	pC28-LIC-sfGFP-His	pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag
165480	pRB30	pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His
165481	pRB30-GFP	pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal uv-GFP, Flag and His
165482	pRB33	pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His
165483	pRB33-GFP	pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal uv-GFP, Flag and His