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Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection.
Graham TGW, Dugast-Darzacq C, Dailey GM, Nguyenla XH, Van Dis E, Esbin MN, Abidi A, Stanley SA, Darzacq X, Tjian R
PLoS One. 2021 Feb 3;16(2):e0246647. doi: 10.1371/journal.pone.0246647. eCollection 2021.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
166944pET-28a_6H-TAQ_E602DBacterial expression of Taq DNA polymerase
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166945pET-28a_6H-MMLV_RT_D524N-6HBacterial expression of M-MLV reverse transcriptase
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