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Generation and application of a versatile CRISPR toolkit for mammalian cell engineering

Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
Synthetic Biology, Volume 6, Issue 1, 2021 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
167856NAX_CAGGS_CAS9_BlastoPiggyBac compatible plasmid expressing spCas9
167857NAX_CAGGS_CAS9_EBFP2PiggyBac compatible plasmid expressing spCas9
167858NAX_CAGGS_CAS9_EYFPPiggyBac compatible plasmid expressing spCas9
167859NAX_CAGGS_CAS9_HygroPiggyBac compatible plasmid expressing spCas9
167860NAX_CAGGS_CAS9_iRFP713PiggyBac compatible plasmid expressing spCas9
167861NAX_CAGGS_CAS9_KanR neoPiggyBac compatible plasmid expressing spCas9
167862NAX_CAGGS_CAS9_mcherryPiggyBac compatible plasmid expressing spCas9
167863NAX_CAGGS_CAS9_PuroPiggyBac compatible plasmid expressing spCas9
167864NAX_CAGGS_CAS9_m4_BlastoPiggyBac compatible plasmid expressing dCas9
167865NAX_CAGGS_CAS9_m4_EBFP2PiggyBac compatible plasmid expressing dCas9
167866NAX_CAGGS_CAS9_m4_EYFPPiggyBac compatible plasmid expressing dCas9
167867NAX_CAGGS_CAS9_m4_HygroPiggyBac compatible plasmid expressing dCas9
167868NAX_CAGGS_CAS9_m4_iRFP713PiggyBac compatible plasmid expressing dCas9
167869NAX_CAGGS_CAS9_m4_KanR neoPiggyBac compatible plasmid expressing dCas9
167870NAX_CAGGS_CAS9_m4_mcherryPiggyBac compatible plasmid expressing dCas9
167871NAX_CAGGS_CAS9_m4_PuroPiggyBac compatible plasmid expressing dCas9
167872NAX_CAGGS_CAS9_m4_KRAB_BlastoPiggyBac compatible plasmid expressing dCas9-KRAB
167873NAX_CAGGS_CAS9_m4_KRAB_EBFP2PiggyBac compatible plasmid expressing dCas9-KRAB
167874NAX_CAGGS_CAS9_m4_KRAB_EYFPPiggyBac compatible plasmid expressing dCas9-KRAB
167875NAX_CAGGS_CAS9_m4_KRAB_HygroPiggyBac compatible plasmid expressing dCas9-KRAB
167876NAX_CAGGS_CAS9_m4_KRAB_iRFP713PiggyBac compatible plasmid expressing dCas9-KRAB
167877NAX_CAGGS_CAS9_m4_KRAB_KanR neoPiggyBac compatible plasmid expressing dCas9-KRAB
167878NAX_CAGGS_CAS9_m4_KRAB_mcherryPiggyBac compatible plasmid expressing dCas9-KRAB
167879NAX_CAGGS_CAS9_m4_KRAB_PuroPiggyBac compatible plasmid expressing dCas9-KRAB
167881NAX_CAGGS_CAS9_m4_VPR_EBFP2PiggyBac compatible plasmid expressing dCas9-VPR
167882NAX_CAGGS_CAS9_m4_VPR_EYFPPiggyBac compatible plasmid expressing dCas9-VPR
167883NAX_CAGGS_CAS9_m4_VPR_HygroPiggyBac compatible plasmid expressing dCas9-VPR
167884NAX_CAGGS_CAS9_m4_VPR_iRFP713PiggyBac compatible plasmid expressing dCas9-VPR
167885NAX_CAGGS_CAS9_m4_VPR_KanR neoPiggyBac compatible plasmid expressing dCas9-VPR
167886NAX_CAGGS_CAS9_m4_VPR_mcherryPiggyBac compatible plasmid expressing dCas9-VPR
167887NAX_CAGGS_CAS9_m4_VPR_PuroPiggyBac compatible plasmid expressing dCas9-VPR
167888NAX_CAGGS_CAS9_VPR_BlastoPiggyBac compatible plasmid expressing Cas9-VPR
167889NAX_CAGGS_CAS9_VPR_EBFP2PiggyBac compatible plasmid expressing Cas9-VPR
167890NAX_CAGGS_CAS9_VPR_EYFPPiggyBac compatible plasmid expressing Cas9-VPR
167891NAX_CAGGS_CAS9_VPR_HygroPiggyBac compatible plasmid expressing Cas9-VPR
167892NAX_CAGGS_CAS9_VPR_iRFP713PiggyBac compatible plasmid expressing Cas9-VPR
167893NAX_CAGGS_CAS9_VPR_KanR neoPiggyBac compatible plasmid expressing Cas9-VPR
167894NAX_CAGGS_CAS9_VPR_mcherryPiggyBac compatible plasmid expressing Cas9-VPR
167895NAX_CAGGS_CAS9_VPR_PuroPiggyBac compatible plasmid expressing Cas9-VPR
167896NAX_CAGGS_CAS9_m4_KRAB-MeCP2_BlastoPiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid
167897NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid
167898NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFPPiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid
167899NAX_CAGGS_CAS9_m4_KRAB-MeCP2_HygroPiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid
167901NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neoPiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid
167902NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherryPiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid
167903NAX_CAGGS_CAS9_m4_KRAB-MeCP2_PuroPiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid
167904pSB700 lenti gRNA BlastoLentiviral vector for expressing U6 sgRNA cloning plasmid
167905pSB700 lenti gRNA EBFP2Lentiviral vector for expressing U6 sgRNA cloning plasmid
167906pSB700 lenti gRNA EYFPLentiviral vector for expressing U6 sgRNA cloning plasmid
167907pSB700 lenti gRNA HygroLentiviral vector for expressing U6 sgRNA cloning plasmid
167908pSB700 lenti gRNA iRFP713Lentiviral vector for expressing U6 sgRNA cloning plasmid
167909pSB700 lenti gRNA KanR neoLentiviral vector for expressing U6 sgRNA cloning plasmid
167910pSB700 lenti gRNA mcherryLentiviral vector for expressing U6 sgRNA cloning plasmid
167911pSB700 lenti gRNA PuroLentiviral vector for expressing U6 sgRNA cloning plasmid
167912pSB700 lenti gRNA Blasto_zhang2.0Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid
167914pSB700 lenti gRNA EYFP_zhang2.0Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid
167915pSB700 lenti gRNA Hygro_zhang2.0Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid
167916pSB700 lenti gRNA iRFP713_zhang2.0Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid
167917pSB700 lenti gRNA KanR neo_zhang2.0Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid
167919pSB700 lenti gRNA Puro_zhang2.0Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid
167920pSB700 lenti gRNA Blasto_TtoC and hairpin extensionLentiviral vector for expressing U6 FE-sgRNA cloning plasmid
167921pSB700 lenti gRNA EBFP2_TtoC and hairpin extensionLentiviral vector for expressing U6 FE-sgRNA cloning plasmid
167922pSB700 lenti gRNA EYFP_TtoC and hairpin extensionLentiviral vector for expressing U6 FE-sgRNA cloning plasmid
167923pSB700 lenti gRNA Hygro_TtoC and hairpin extensionLentiviral vector for expressing U6 FE-sgRNA cloning plasmid
167924pSB700 lenti gRNA iRFP713_TtoC and hairpin extensionLentiviral vector for expressing U6 FE-sgRNA cloning plasmid
167925pSB700 lenti gRNA KanR neo_TtoC and hairpin extensionLentiviral vector for expressing U6 FE-sgRNA cloning plasmid
167927pSB700 lenti gRNA Puro_TtoC and hairpin extensionLentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Antibodies from Article