Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp14/nsp10 exoribonuclease.
Canal B, McClure AW, Curran JF, Wu M, Ulferts R, Weissmann F, Zeng J, Bertolin AP, Milligan JC, Basu S, Drury LS, Deegan TD, Fujisawa R, Roberts EL, Basier C, Labib K, Beale R, Howell M, Diffley JFX
Biochem J. 2021 Jul 16;478(13):2445-2464. doi: 10.1042/BCJ20210198. PubMed Article
Canal B, McClure AW, Curran JF, Wu M, Ulferts R, Weissmann F, Zeng J, Bertolin AP, Milligan JC, Basu S, Drury LS, Deegan TD, Fujisawa R, Roberts EL, Basier C, Labib K, Beale R, Howell M, Diffley JFX
Biochem J. 2021 Jul 16;478(13):2445-2464. doi: 10.1042/BCJ20210198. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169157 | pET11a_3xFlag-nsp10 (SARS-CoV-2) | To express SARS-CoV-2 nsp10 in E. coli |
| 169158 | pCOLADuet-1_nsp10 (SARS-CoV-2) | To express SARS-CoV-2 nsp10 in E. coli |
| 169159 | pET11a_3xFlag-nsp14 (SARS-CoV-2) | To express SARS-CoV-2 nsp14 in E. coli |
| 169160 | pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2) | To express nsp14-nsp10 fusion protein in E. coli |
| 169161 | pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2) | To express nsp10-nsp14 fusion protein in E. coli |
| 169162 | pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2) | Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells |
| 169163 | pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2) | Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells |
| 169164 | pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2) | Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells |