A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines.

Eggenschwiler R, Gschwendtberger T, Felski C, Jahn C, Langer F, Sterneckert J, Hermann A, Luhmann J, Steinemann D, Haase A, Martin U, Petri S, Cantz T
Sci Rep. 2021 Nov 12;11(1):22154. doi: 10.1038/s41598-021-01689-2. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
162796	PB-PE	All-in-one prime editor piggyBac transposon
162797	PB-PE VQR	All-in-one prime editor piggyBac transposon, VQR variant
172652	PB-PE xCas9	All-in-one prime editor piggyBac transposon (xCas9)
174554	PB-PE NG	All-in-one prime editor piggyBac transposon, NG variant
179081	PB-PE SpG	NOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variant
179082	PB-PE SpRY	NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant
187893	PB-PEmax	All-in-one prime editor piggyBac transposon with PEmax
187894	PB-PEmax NG	All-in-one prime editor piggyBac transposon with PEmax, NG variant
187895	PB-PEmax SpG	All-in-one prime editor piggyBac transposon with PEmax, SpG variant