Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn MP, Unruh JR, Florens L, Conaway RC, Conaway JW
Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 10.1073/pnas.2107277118. PubMed Article
Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn MP, Unruh JR, Florens L, Conaway RC, Conaway JW
Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 10.1073/pnas.2107277118. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 174792 | pET28_6xHis-PARP1-C125G | Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break) |
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| 174793 | pET28_6xHis-PARP1-W318R | Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding) |
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| 174794 | pET28_6xHis-PARP1-L713F | Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain) |
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| 174795 | pET28_6xHis-PARP1-A762V | Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism) |
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| 174796 | pET28_6xHis-PARP1-A774L | Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain) |
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| 174797 | pET28_6xHis-PARP1-A870L | Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain) |
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| 174798 | pET28_6xHis-PARP1-G871L | Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain) |
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| 174799 | pET28_6xHis-PARP1-K893I | Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding) |
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| 174800 | pET28_6xHis-PARP1-G972R | Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall |
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| 174801 | pET28_6xHis-PARP1-Y986H | Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall |
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| 174802 | pET28_6xHis-PARP1-Y986S | Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers |
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| 174804 | pET28_6xHis-PARP1deltaART | Bacterial expression of an inactive PARP1 mutant lacking the ART subdomain |
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| 174805 | pET28_6xHis-PARP1-Zn2 | Bacterial expression of isolated PARP1 Zn2 domain |
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| 174806 | pET28_6xHis-PARP1-Zn3 | Bacterial expression of isolated PARP1 Zn3 domain |
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| 174807 | pET28_6xHis-PARP1-BRCT | Bacterial expression of isolated PARP1 BRCT domain |
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| 174808 | pET28_6xHis-PARP1-WGR | Bacterial expression of isolated PARP1 WGR domain |
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| 174809 | pET28_6xHis-PARP1-HD | Bacterial expression of isolated PARP1 HD subdomain |
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| 174810 | pET28_6xHis-PARP1-ART | Bacterial expression of isolated PARP1 ART subdomain |
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| 174811 | pET28_6xHis-PARP1-CATdeltaHD | Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain |
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| 174813 | pET41sIII_GST-ALC1-K77R | Bacterial expression of inactive ALC1 (K77R disrupts ATP binding) |
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| 174814 | pET41sIII_GST-ALC1-E175Q | Bacterial expression of inactive ALC1 (E175Q disrupts ATP binding) |
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