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In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures.
Alfadil E, Bradke F, Dupraz S
J Vis Exp. 2021 Oct 14;(176). doi: 10.3791/63068. PubMed Article

Plasmids from Article

ID Plasmid Purpose
175257pCAG-loxpSTOPloxp-lyn-NeonGreenmembrane target protein labelling
175258pTub-alpha1-iLifeact-NeonGreenF-actin structures labelling
175259pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreendual plasmid labelling membrane target protein of different neuronal population with different flurophore
175261pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherrylabelling of microtubule plus end and F-actin structures
175263pTub-alpha-EB3-NeonGreenlabelling of microtubule plus end and F-actin structures
175437pTub-alpha1-Lifeact-GFPF-actin structures labelling
175438pCAG-lox-rox-STOP-rox-tRFP-pA-lox-ZsGreen-pAdual plasmid labelling cells of different neuronal population with different fluorophores

Antibodies from Article