Addgene: Sulfadiazine and phosphinothricin selection systems optimised for the transformation of tobacco BY-2 cells.

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Lukas Fischer
Kobercova et al

Sulfadiazine and phosphinothricin selection systems optimised for the transformation of tobacco BY-2 cells.
Kobercova E, Srba M, Fischer L
Plant Cell Rep. 2023 Jan 7. doi: 10.1007/s00299-022-02975-7. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
186423	pUPD2_SulR(CDS)	provides sulfadiazine resistance (sulI) as a level 0 GoldenBraid part (CDS; B3-B4-B5)
186424	pDGB1alpha11_SulR	transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assembly
186425	pDGB1alpha2_SulR	transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter
186426	pDGB3omega1_KanR_BastaR	optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)
186427	pDGB1alpha2_KanR_BastaR_Rb7_GFP	optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

Antibodies from Article