A Comprehensive Roadmap Towards the Generation of an Influenza B Reporter Assay Using a Single DNA Polymerase-Based Cloning of the Reporter RNA Construct.
Kedia N, Banerjee S, Mondal A
Front Microbiol. 2022 May 25;13:868367. doi: 10.3389/fmicb.2022.868367. eCollection 2022. PubMed Article
Kedia N, Banerjee S, Mondal A
Front Microbiol. 2022 May 25;13:868367. doi: 10.3389/fmicb.2022.868367. eCollection 2022. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187916 | pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008 | Expresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells |
| 187917 | pcDNA3-PB2-B/BRISBANE/60/2008 | Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells |
| 187918 | pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008 | Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells |
| 187919 | pcDNA3-PB1-B/BRISBANE/60/2008 | Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells |
| 187920 | pcDNA3-PA-B/BRISBANE/60/2008 | Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells |
| 187921 | pcDNA3-NP-B/BRISBANE/60/2008 | Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells |
| 187922 | pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008 | Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells |
| 187923 | pcDNA3-NP-V5-B/BRISBANE/60/2008 | Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells |
| 187924 | pHH21-BNA-Luc | Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells |