PurposeExpresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||187919||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5504
- Total vector size (bp) 7763
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsStreak an ampicillin agar plate from the frozen glycerol stock. Grow bacterial culture from a single colony grown on the plate.
Copy numberHigh Copy
Gene/Insert namepolymerase basic protein 1
Insert Size (bp)2259
- Promoter CMV Promoter
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-PB1-B/BRISBANE/60/2008 was a gift from Arindam Mondal (Addgene plasmid # 187919 ; http://n2t.net/addgene:187919 ; RRID:Addgene_187919)
For your References section:A Comprehensive Roadmap Towards the Generation of an Influenza B Reporter Assay Using a Single DNA Polymerase-Based Cloning of the Reporter RNA Construct. Kedia N, Banerjee S, Mondal A. Front Microbiol. 2022 May 25;13:868367. doi: 10.3389/fmicb.2022.868367. eCollection 2022. 10.3389/fmicb.2022.868367 PubMed 35694292