An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria.

Greenwich JL, Alakavuklar MA, Fuqua C
Microbiol Resour Announc. 2023 Feb 16;12(2):e0111922. doi: 10.1128/mra.01119-22. Epub 2023 Jan 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
192979	pMAT36	BHR plasmid (pRA301) expressing mirA-3XFLAG from T7 promoter for high yield protein purification
192980	pJLG029	BHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purification
192981	pJLG038	BHR plasmid (pBBR1 ori) expressing T7 polymerase under control of the lac promoter
192982	pJLG039	BHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoter