Enhancing Sox/Oct cooperativity induces higher-grade developmental reset
MacCarthy CM, Malik V, Wu G, Velychko T, Keshet G, Jauch R, Cojocaru V, Schöler HR, Velychko S
bioRxiv 2022.09.23.509242 Article
MacCarthy CM, Malik V, Wu G, Velychko T, Keshet G, Jauch R, Cojocaru V, Schöler HR, Velychko S
bioRxiv 2022.09.23.509242 Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 193290 | pCXLE-SOX2-17-p2a-KLF4 | episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion |
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| 193291 | pCXLE-SOX2AV-p2a-KLF4 | episomal expressing human SOX2A61V + KLF4 |
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| 193292 | pCXLE-SOX2-p2a-KLF4 | episomal hSK (expressing human SOX2 + KLF4) |
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| 193293 | pCXLE-mCherry | episomal vector expressing mCherry fluorescent protein |
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| 193294 | pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4 | episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion |
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| 193295 | pCXLE-mCherry-t2a-SOX2AV-p2a-KLF4 | episomal vector expressing mCherry + human SOX2A61V + KLF4 |
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| 193296 | pCXLE-mCherry-t2a-SOX2-p2a-KLF4 | episomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4) |
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| 193298 | pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17 | episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs |
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| 193299 | pHAGE2-TetOminiCMV-Klf4-IRES-Sox2-17 | tetO-KS* (Dox-inducible reprogramming lentiviral vector expressing mouse Klf4 + super-Sox) |
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| 193345 | pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4 | tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox) |
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| 193346 | pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc | tetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + super-Sox + Klf4 + cMyc) |
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| 193347 | pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc | tetO-Sox2AV-KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Sox2A61V + Klf4 + cMyc) |
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| 193348 | pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4 | tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4) |
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| 193349 | pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc | tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc) |
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| 193350 | pMXs-hSOX2-17 | Retroviral vector expressing human SOX2-17 chimeric transcription factor (super-SOX) |
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| 193351 | pMXs-hSOX2 | Retroviral vector expressing human SOX2 |
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| 193352 | pMXs-hSOX2AV | Retroviral vector expressing human SOX2A61V point mutant |
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| 193353 | pMXs-Sox2-17 | Retroviral vector expressing mouse Sox2-17 chimeric transcription factor (super-Sox) |
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| 193354 | pMXs-Sox2AV | Retroviral vector expressing mouse Sox2A61V point mutant |
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| 193355 | T7-VEE-mCherry | Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR |
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| 193356 | T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 | VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs |
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| 193357 | T7-VEE-CFP | Self-replicating RNA vector expressing CFP fluorescent protein + PuroR |
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| 193358 | T7-VEE-CFP-e2a-SOX2-p2a-KLF4 | VEE-CFP-SK (self-replicating RNA vector expressing CFP + human SOX2 + KLF4 + PuroR) |
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| 193359 | T7-VEE-CFP-e2a-SOX2AV-p2a-KLF4 | self-replicating RNA vector expressing CFP + human SOX2A61V + KLF4 + PuroR |
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| 193360 | T7-VEE-CFP-e2a-SOX2-17-p2a-KLF4 | VEE-CFP-S*K (self-replicating RNA vector expressing CFP + human super-SOX + KLF4 + PuroR) for integration-free naïve conversion |
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