Programmable protein delivery with a bacterial contractile injection system.
Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F
Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198271 | pAWP78-PVCpnf1-16 | Unmodified pPVC; contains WT PVC structural/accessory genes |
| 198272 | pBR322-PVCpnf17-22 | Unmodified pPayload; contains WT PVC payloads/regulatory genes |
| 198273 | pBR322-PVCpnf18-21 | Contains only regulatory genes; for generating empty PVCs |
| 198274 | pBR322-Pdp1_NTD-Cre | For loading Cre into PVCs; also contains PVC regulatory genes |
| 198275 | pBR322-Pdp1_NTD-Cas9 | For loading SpCas9 into PVCs; also contains PVC regulatory genes |
| 198276 | pBR322-Pdp1_NTD-ZFD_L | For loading L half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Left-G1397-N_DddA); also contains PVC regulatory genes |
| 198277 | pBR322-Pdp1_NTD-ZFD_R | For loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genes |
| 198278 | pIEx4-Dio-GFP | Cre reporter for insect cells; double-floxxed-EGFP |
| 198279 | pcDNA3-Dio-GFP | Cre reporter for mammalian cells; double-floxxed-EGFP |
| 198280 | pIEx4-Cre | Expresses Cre in insect cells |
| 198281 | pcDNA3-Cre | Expresses Cre in mammalian cells |
| 198282 | py100-sgRNA_hVEGFA | Expression of SpCas9 guide RNA specific for human VEGFA locus |
| 198283 | pAWP78-PVCpnfΔpvc13 | Produces PVCs lacking tail fibers |
| 198284 | pAWP78-PVCpnfΔpvc15 | Produces PVCs lacking payloads by elimination of the payload loader (pvc15) |
| 198285 | pAWP78-PVCpnf_FLAG-pvc2 | Contains an external epitope tag on the sheath protein; for immunofluorescence-based binding assays |
| 198286 | pAWP78-PVCpnf_pvc13-truncated | Contains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCs |
| 198287 | pAWP78-PVCpnf_pvc13-E01DARPin | Produces PVCs retargeted with anti-EGFR DARPin E01 |
| 198288 | pAWP78-PVCpnf_pvc13-A4DARPin | Produces PVCs retargeted with anti-lysozyme DARPin A4 |
| 198289 | pAWP78-PVCpnf_pvc13-Ad5Knob | Produces PVCs retargeted with knob domain from hAd5 virus |
| 198290 | pAWP78-PVCpnf_pvc13-Ad5DL491-492 | Produces PVCs retargeted with non-binding mutant of Ad5 knob |
| 198291 | pAWP78-PVCpnf_pvc13-Ad5RGDPK7 | Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions) |
| 198292 | pAWP78-PVCpnf_pvc13-antiMouseMHCIINb | Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody |
| 198293 | pAWP78-PVCpnf_pvc13-antiHistoneNb | Produces PVCs retargeted with A9 anti-histone nanobody |
| 198294 | pAWP78-PVCpnf_pvc13-CD4DARPin | Produces PVCs retargeted with anti-CD4 DARPin D27.2 |
| 198295 | pAWP78-PVCpnf_pvc13-CD11bDARPin | Produces PVCs retargeted with anti-CD11b DARPin F7 |
| 198296 | pAWP78-PVCpnf_pvc13-HA | Produces PVCs retargeted with HA tag |
| 198297 | pAWP78-PVCpnf_pvc13-FLAG | Produces PVCs retargeted with FLAG tag |
| 198298 | pAWP78-PVCpnf_pvc13-EE | Produces PVCs retargeted with EE tag |
| 198299 | pAWP78-PVCpnf_pvc13-MoonTag | Produces PVCs retargeted with MoonTag |
| 198300 | pAWP78-PVCpnf_pvc13-SunTag | Produces PVCs retargeted with SunTag |
| 198301 | pAWP78-PVCpnf_pvc13-ALFA | Produces PVCs retargeted with ALFA tag |
| 198302 | pHCMV-SS-antiHAscFv-TM | Displays anti-HA scFv from the surface of mammalian cells |
| 198303 | pHCMV-SS-antiFLAGscFv-TM | Displays anti-FLAG scFv from the surface of mammalian cells |
| 198304 | pHCMV-SS-antiEEscFv-TM | Displays anti-EE scFv from the surface of mammalian cells |
| 198305 | pHCMV-SS-antiMoonTagNb-TM | Displays anti-MoonTag nanobody from the surface of mammalian cells |
| 198306 | pHCMV-SS-antiSunTag-TM | Displays anti-SunTag scFv from the surface of mammalian cells |
| 198307 | pHCMV-SS-antiALFAtagNb-TM | Displays anti-ALFA nanobody from the surface of mammalian cells |
| 198308 | pAWP78-PVCpnf_pvc12-HiBiT | Produces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assays |
| 198309 | pAWP78-PVCpnfΔpvc15_pvc12-HiBiT | Negative control for payload loading assay; produces PVCs deficient for payload loader (pvc15) |
| 198310 | pBR322-Pdp1-HiBiT | Loads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains Pnf |
| 198311 | pBR322-Pdp1_aa1-289-HiBiT | Loads a truncated payload; for identification of Pdp1 packaging domain |
| 198312 | pBR322-Pdp1_aa1-190-HiBiT | Loads a truncated payload; for identification of Pdp1 packaging domain |
| 198313 | pBR322-Pdp1_aa1-133-HiBiT | Loads a truncated payload; for identification of Pdp1 packaging domain |
| 198314 | pBR322-Pdp1_aa1-76-HiBiT | Loads a truncated payload; for identification of Pdp1 packaging domain |
| 198315 | pBR322-Pdp1_aa1-38-HiBiT | Loads a truncated payload; for identification of Pdp1 packaging domain |
| 198316 | pBR322-Pdp1_aa39-331-HiBiT | Loads a truncated payload; for identification of Pdp1 packaging domain |
| 198317 | pBR322-Pdp1_NTD-GFP-HiBiT | Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf |
| 198318 | pBR322-Pdp1_NTD-Cre-HiBiT | Loads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains Pnf |
| 198319 | pBR322-Pdp1_NTD-ZFN_L-HiBiT | Loads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domain |
| 198320 | TwinStrep-SUMO-Pdp1 | For affinity purification of Pdp1 payload |
| 198321 | TwinStrep-SUMO-Pnf | For affinity purification of Pnf payload |
| 198448 | pAWP78-PVCpnf_Δpvc10 | Produces PVCs containing truncated spike tip protein (Pvc10). |
| 198449 | pAWP78-PVCpnf_pvc13-Ad5RGDPK7_Δpvc10 | Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions); also contains truncated spike tip protein (Pvc10). |
| 198450 | pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10 | Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10). |