A nanobody-based strategy for rapid and scalable purification of human protein complexes.
Stevens TA, Tomaleri GP, Hazu M, Wei S, Nguyen VN, DeKalb C, Voorhees RM, Pleiner T
Nat Protoc. 2024 Jan;19(1):127-158. doi: 10.1038/s41596-023-00904-w. Epub 2023 Nov 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199346 | pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS | Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells. |
| 199347 | pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS | Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells. |
| 199348 | pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS | Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells. |
| 199349 | pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS | Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells. |
| 199350 | pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS | Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells. |
| 199351 | pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS | Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells. |
| 199352 | pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS | Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells. |
| 199353 | pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS | Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells. |
| 199354 | pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS | Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells. |
| 199355 | pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS | Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells. |
| 199356 | pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells. |
| 199357 | pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells. |
| 199358 | pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells. |
| 199359 | pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells. |
| 199360 | pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells. |
| 199361 | pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells. |
| 199362 | pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells. |
| 199363 | pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells. |
| 199364 | pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells. |
| 199365 | pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells. |
| 199366 | pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells. |
| 199367 | pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells. |
| 199368 | pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells. |
| 199369 | pTS116 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-Alfa-P2A-TagBFP | Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells. |
| 199370 | pTS117 His14-Avi-45xGS-anti GFP nanobody | Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi) |
| 199371 | pTS118 His14-Avi-28xGS-anti ALFA tag nanobody | Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi) |
| 199390 | pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody | Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag |
| 199391 | pTP341 pHAGE2 CMV TagBFP-3xFLAG | Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells |
| 199392 | pTP924 pHAGE2 CMV GFP | Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells |