A massively parallel reporter assay library to screen short synthetic promoters in mammalian cells.
Zahm AM, Owens WS, Himes SR, Fallon BS, Rondem KE, Gormick AN, Bloom JS, Kosuri S, Chan H, English JG
Nat Commun. 2024 Nov 28;15(1):10353. doi: 10.1038/s41467-024-54502-9.
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Article
These individual pooled libraries are also available as a kit: TRE-MPRA full library kit
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201012 | TRE-MPRA_minCMV Library | Massively parallel reporter assay (MPRA) library with minCMV promoter |
| 201013 | TRE-MPRA_minPromega Library | Massively parallel reporter assay (MPRA) library with minPromega promoter |
| 201014 | TRE-MPRA_minTK Library | Massively parallel reporter assay (MPRA) library with minTK promoter |
| 211516 | pGL4.R_TRE_minTK | Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression |
| 211517 | pGL4.R_TRE_minPro | Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression |
| 211518 | pGL4.R_TRE_minCMV | Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression |