microTRE Screening Libraries
(Pooled Libraries #201012, #201013, #201014, #1000000220)
A massively parallel reporter assay (MPRA) library where barcode expression is dependent upon engagement of cellular transcription factors to a series of engineered, short, artificial transcriptional response elements (TREs) encoded alongside variable motifs and minimal promoters.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||201012||TRE-MPRA_minCMV library||1||$ 640||Add to Cart|
|Pooled Library||201013||TRE-MPRA_minPromega library||1||$ 640||Add to Cart|
|Pooled Library||201014||TRE-MPRA_minTK library||1||$ 640||Add to Cart|
|Pooled Library||1000000220||TRE-MPRA full library kit
||1||$ 1600||Add to Cart|
SpeciesSynthetic, but based on human and mouse
Unique barcodesEach of the three pooled libraries contains approximately 1.2 × 106 unique barcodes mapping to the TRE-barcode dictionary.
Insert size range59–148 bp
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Terms and LicensesAcademic/Nonprofit Terms
The Pooled NGS data provided by the depositor is from representative preps, and library composition changes very little between lots. NGS sequencing is required for the analysis pipeline, and datasets provided from the depositor can be used for comparative reference.
The necessary algorithms and barcode dictionary spreadsheet can be found on GitHub (Link opens in a new window).
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:microTRE Screening library was a gift from Justin English (Addgene #)
For your References section:Discovery and Validation of Context-Dependent Synthetic Mammalian Promoters. Zahm AM, Owens WS, Himes SR, Rondem KE, Fallon BS, Gormick AN, Bloom JS, Kosuri S, Chan H, English JG. bioRxiv. 2023 May 11:2023.05.11.539703. doi: 10.1101/2023.05.11.539703. Preprint. PubMed 37214829