A multiplex method for rapidly identifying viral protease inhibitors.
Hong SJ, Resnick SJ, Iketani S, Cha JW, Albert BA, Fazekas CT, Chang CW, Liu H, Dagan S, Abagyan MR, Fajtova P, Culbertson B, Brace B, Reddem ER, Forouhar F, Glickman JF, Balkovec JM, Stockwell BR, Shapiro L, O'Donoghue AJ, Sabo Y, Freundlich JS, Ho DD, Chavez A
Mol Syst Biol. 2025 Feb;21(2):158-172. doi: 10.1038/s44320-024-00082-1. Epub 2025 Jan 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201792 | pAG416GAL-EYFP | Expresses EYFP from a GAL promoter with a URA3 marker |
| 201935 | pAG416GAL-1x-HIV-2_PR | Expresses HIV-2 protease from a GAL-1x promoter with a URA3 marker |
| 201936 | pAG416GAL-2x-uA-BWCoV-SW1_3CL | Expresses Beluga Whale-CoV SW1 3CL protease from a GAL-2x promoter with a URA3 marker |
| 201937 | pAG416GAL-3x-uC-HCoV-229E_3CL | Expresses HCoV-229E 3CL protease from a GAL-3x promoter with a URA3 marker |
| 201938 | pAG416GAL-3x-uC-HCoV-NL63_3CL | Expresses HCoV-NL63 3CL protease from a GAL-3x promoter with a URA3 marker |
| 201939 | pAG416GAL-Bat-SL-CoVZC45_PLP | Expresses Bat-SL-CoVZC45 papain-like protease from a GAL promoter with a URA3 marker |
| 201940 | pAG416GAL-DENV_NS3-GS-NS2B | Expresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker |
| 201941 | pAG416GAL-EMCV_3C | Expresses EMCV 3C protease from a GAL promoter with a URA3 marker |
| 201942 | pAG416GAL-SARS-CoV_PLP | Expresses SARS-CoV papain-like protease from a GAL promoter with a URA3 marker |
| 201943 | pAG416GAL10-Bat-CoV-HKU4_3CL | Expresses Bat-CoV HKU4 3CL protease from a GAL10 promoter with a URA3 marker |
| 201944 | pAG416GAL10-Bat-SL-CoVZC45_3CL | Expresses Bat-SL-CoVZC45 3CL protease from a GAL10 promoter with a URA3 marker |
| 201945 | pAG416GAL10-CCHFV_OTU | Expresses CCHFV OTU domain of L protein from a GAL10 promoter with a URA3 marker |
| 203462 | pAG416GAL10-c-Src | Expresses human c-Src kinase from a GAL10 promoter with a URA3 marker |
| 203463 | pAG416GAL10-DUGV_OTU | Expresses DUGV OTU domain of L protein from a GAL10 promoter with a URA3 marker |
| 203465 | pAG416GAL10-HPeV_3C | Expresses HPeV 3C protease from a GAL10 promoter with a URA3 marker |
| 203466 | pAG416GAL10-HRV-B14_3C | Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker |
| 203467 | pAG416GAL10-HTLV_PR | Expresses HTLV protease from a GAL10 promoter with a URA3 marker |
| 203468 | pAG416GAL10-LGTV_NS2B-GS-NS3 | Expresses LGTV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker |
| 203470 | pAG416GAL10-NWV_3CL | Expresses NWV 3CL protease from a GAL10 promoter with a URA3 marker |
| 203471 | pAG416GAL10-POWV_NS2B-GS-NS3 | Expresses POWV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker |
| 203472 | pAG416GAL10-PV_3C | Expresses PV 3C protease from a GAL10 promoter with a URA3 marker |
| 203473 | pAG416GAL10-SHV_3CL | Expresses SHV 3CL protease from a GAL10 promoter with a URA3 marker |
| 203474 | pAG416GAL10-SV-A_3C | Expresses SV-A 3C protease from a GAL10 promoter with a URA3 marker |
| 203475 | pAG416GAL10-WNV_NS2B-GS-NS3 | Expresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker |
| 203476 | pAG416GAL10-YES1-6stop | Expresses human YES1 kinase from a GAL10 promoter with a URA3 marker |
| 203477 | pAG416GAL10-ZIKV_NS2B-GS-NS3 | Expresses ZIKV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker |
| 203478 | pAG416GALL-Bat-CoV-HKU9_3CL | Expresses Bat-CoV HKU9 3CL protease from a GALL promoter with a URA3 marker |
| 203481 | pAG416GALL-HAV_3C | Expresses HAV 3C protease from a GALL promoter with a URA3 marker |
| 203493 | pAG416GALL-HIV-1_PR | Expresses HIV-1 protease from a GALL promoter with a URA3 marker |
| 203495 | pAG416GALL-SARS-CoV_3CL | Expresses SARS-CoV 3CL protease from a GALL promoter with a URA3 marker |
| 203496 | pAG416GALL-SARS-CoV-2_3CL | Expresses SARS-CoV-2 3CL protease from a GALL promoter with a URA3 marker |
| 203499 | pAG426GAL-CV-B3_3C | Expresses CV-B3 3C protease from a GAL promoter with a URA3 marker |
| 203501 | pAG426GAL-FES | Expresses human FES kinase from a GAL promoter with a URA3 marker |
| 203502 | pAG426GAL-HCoV-NL63_PLP2 | Expresses HCoV-NL63 PLP2 from a GAL promoter with a URA3 marker |
| 203503 | pAG426GAL-HCoV-OC43_3CL | Expresses HCoV-OC43 3CL protease from a GAL promoter with a URA3 marker |
| 203504 | pAG426GAL-HCV_NS3-GS-NS4A | Expresses HCV NS3-NS4A protease (with GS linker) from a GAL promoter with a URA3 marker |
| 203505 | pAG426GAL-HHV-3_PR | Expresses protease domain of HHV-3 (VZV) from a GAL promoter with a URA3 marker |
| 203506 | pAG426GAL-KUNV_NS2B-GS-NS3 | Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker |
| 203507 | pAG426GAL-LV_3CL | Expresses LV 3CL protease from a GAL promoter with a URA3 marker |
| 203508 | pAG426GAL-MERS-CoV_PLP | Expresses MERS-CoV papain-like protease from a GAL promoter with a URA3 marker |
| 203509 | pAG426GAL-SARS-CoV-2_PLP | Expresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 marker |
| 203510 | pAG426GAL-SLEV_NS2B-GS-NS3 | Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker |
| 203511 | pAG416GALL-ccdB-6stop | Gateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 marker |
| 203512 | pAG416GAL10-ccdB-6stop | Gateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 marker |
| 203514 | pAG416GAL-3x-uC-ccdB-6stop | Gateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker |
| 203517 | pAG416GAL-2x-uA-ccdB-6stop | Gateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker |
| 203519 | pAG416GAL-1x-ccdB-6stop | Gateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 marker |