Chromosomal barcodes for simultaneous tracking of near-isogenic bacterial strains in plant microbiota.
Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, Garrido-Oter R, Schulze-Lefert P
Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 10.1038/s41564-024-01619-8. Epub 2024 Mar 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202210 | pBCC075 | Level1 DNA-Tag with bc063 |
| 202211 | pBCC077 | Level1 DNA-Tag with bc065 |
| 202212 | pBCC078 | Level1 DNA-Tag with bc066 |
| 202213 | pBCC086 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum |
| 202214 | pBCC087 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Gentamicin selectable marker and AmCyan1 fluorescent marker |
| 202215 | pBCC088 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker |
| 202216 | pBCC089 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Gentamicin selectable marker and tagBFP fluorescent marker |
| 202217 | pBCC090 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent marker |
| 202218 | pBCC091 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum |
| 202219 | pBCC092 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and AmCyan1 fluorescent marker |
| 202220 | pBCC093 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and tagRFP fluorescent marker |
| 202221 | pBCC095 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and GFP fluorescent marker |
| 202222 | pBCC096 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker |
| 202223 | pBCC097 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and tagBFP fluorescent marker |
| 202224 | pBCC098 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and AmCyan1 fluorescent marker |
| 202225 | pBCC099 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and tagRFP fluorescent marker |
| 202226 | pBCC100 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlum |
| 202227 | pBCC101 | Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and tagBFP fluorescent marker |
| 202233 | pBC069 | Level 2 for strain-specific barcode DNA tag with bc096 through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker |
| 202234 | pBC070 | Level 2 for strain-specific barcode DNA tag with bc097 through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker |
| 202235 | pBC071 | Level 2 for strain-specific barcode DNA tag with bc098 through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker |
| 202236 | pBC072 | Level 2 for strain-specific barcode DNA tag with bc099 through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker |
| 202237 | pBC073 | Level 2 for strain-specific barcode DNA tag with bc100 through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker |
| 202238 | pBC079 | Level 2 for strain-specific barcode DNA tag with bc106 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent marker |
| 202239 | pBC080 | Level 2 for strain-specific barcode DNA tag with bc107 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent marker |
| 202240 | pBC081 | Level 2 for strain-specific barcode DNA tag with bc108 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent marker |
| 202241 | pBC082 | Level 2 for strain-specific barcode DNA tag with bc109 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent marker |
| 202242 | pBC083 | Level 2 for strain-specific barcode DNA tag with bc110 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent marker |
| 202243 | pBC104 | Level 2 for strain-specific barcode DNA tag with bc131 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum |
| 202244 | pBC105 | Level 2 for strain-specific barcode DNA tag with bc132 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum |
| 202245 | pBC106 | Level 2 for strain-specific barcode DNA tag with bc133 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum |
| 202246 | pBC107 | Level 2 for strain-specific barcode DNA tag with bc134 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum |
| 202247 | pBC108 | Level 2 for strain-specific barcode DNA tag with bc135 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum |
| 202248 | pBC160 | Level 2 for strain-specific barcode DNA tag with bc187 through Tn7 bacterial insertion, Gentamicin selectable marker and AmCyan1 fluorescent marker |
| 202249 | pBC161 | Level 2 for strain-specific barcode DNA tag with bc188 through Tn7 bacterial insertion, Gentamicin selectable marker and AmCyan1 fluorescent marker |
| 202250 | pBC162 | Level 2 for strain-specific barcode DNA tag with bc189 through Tn7 bacterial insertion, Gentamicin selectable marker and AmCyan1 fluorescent marker |
| 202251 | pBC124 | Level 2 for strain-specific barcode DNA tag with bc151 through Tn7 bacterial insertion, Kanamycin selectable marker and GFP fluorescent marker |
| 202252 | pBC125 | Level 2 for strain-specific barcode DNA tag with bc152 through Tn7 bacterial insertion, Kanamycin selectable marker and GFP fluorescent marker |
| 202254 | pBC127 | Level 2 for strain-specific barcode DNA tag with bc154 through Tn7 bacterial insertion, Kanamycin selectable marker and tagRFP fluorescent marker |
| 202255 | pBC128 | Level 2 for strain-specific barcode DNA tag with bc155 through Tn7 bacterial insertion, Kanamycin selectable marker and tagRFP fluorescent marker |
| 202256 | pBC129 | Level 2 for strain-specific barcode DNA tag with bc156 through Tn7 bacterial insertion, Kanamycin selectable marker and tagRFP fluorescent marker |
| 202257 | pBC130 | Level 2 for strain-specific barcode DNA tag with bc157 through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlum |
| 202258 | pBC131 | Level 2 for strain-specific barcode DNA tag with bc158 through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlum |
| 202259 | pBC132 | Level 2 for strain-specific barcode DNA tag with bc159 through Tn7 bacterial insertion, Kanamycin selectable marker and tagBFP fluorescent marker |
| 202260 | pBC133 | Level 2 for strain-specific barcode DNA tag with bc160 through Tn7 bacterial insertion, Kanamycin selectable marker and tagBFP fluorescent marker |
| 202261 | pBC134 | Level 2 for strain-specific barcode DNA tag with bc161 through Tn7 bacterial insertion, Kanamycin selectable marker and tagBFP fluorescent marker |
| 202262 | pBC135 | Level 2 for strain-specific barcode DNA tag with bc162 through Tn7 bacterial insertion, Kanamycin selectable marker and tagBFP fluorescent marker |
| 202274 | pBC155 | Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker |
| 202275 | pBC113 | Level 2 for strain-specific barcode DNA tag with bc140 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum |
| 202276 | pBC114 | Level 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum |
| 202277 | pBC115 | Level 2 for strain-specific barcode DNA tag with bc142 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum |
| 202278 | pBC117 | Level 2 for strain-specific barcode DNA tag with bc144 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker |
| 202279 | pBC118 | Level 2 for strain-specific barcode DNA tag with bc145 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker |
| 202280 | pBC119 | Level 2 for strain-specific barcode DNA tag with bc146 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker |