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Hominoid SVA-lncRNA AK057321 targets human-specific SVA retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal maturation.
Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson M, Moreno A, Matthew P Anderson
Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
202800pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puroLentiviral vector to express SVA-lncRNA AK057321 with ires for mcherry and puro expression
202813pLVX-SVA-lncRNA-AK057321-mcherryLentiviral vector to express of SVA-lncRNA AK057321 with CMV for mcherry expression
202818pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mcherryLentiviral vector to express 5'-aptamer tagged SVA-lncRNA AK057321 with CMV for mcherry expression
202821lentiCRISPRv2-sgRNAs-del-AK057321-SVA-mcherryLentiviral vector modified to express two sgRNAs that delete the SVA within the SVA-lncRNA AK057321 gene (exon 3) with EF-1apha for Cas9 and mcherry expression
202822lentiCRISPRv2-sgRNAs-del-ZNF91-mcherryLentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression
202823lentiCRISPRv2-sgRNAs-del-ZNF91-BFPLentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and BFP expression
202824lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFPLentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression
202825lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFPLentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expression
202826lentiCRISPRv2-sgRNAs-del-SCN8A-SVA-BFPLentiviral vector modified to express two sgRNAs that delete the intronic SVA within the SCN8A gene with EF-1apha for Cas9 and BFP expression
202827pGL3-luciferase-reporter-AGPAT3-SVApGL3 luciferase reporter with AGPAT3 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202828pGL3-luciferase-reporter-CDK5RAP2-SVApGL3 luciferase reporter with CDK5RAP2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202829pGL3-luciferase-reporter-CHAF1B-SVApGL3 luciferase reporter with CHAF1B SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202830pGL3-luciferase-reporter-CHAF1B-SVA-del-VNTRpGL3 luciferase reporter with CHAF1B-SVA-del-VNTR sequence cloned upstream of SV40 promoter to test effects of SVA-del-VNTR insertion on luciferase expression
202831pGL3-luciferase-reporter-POFUT2-SVApGL3 luciferase reporter with POFUT2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202832pGL3-luciferase-reporter-JAM2-SVApGL3 luciferase reporter with JAM2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202833pGL3-luciferase-reporter-HTT-SVApGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202834pLVX-SVA-lncRNA-AK057321 shRNA 1ALentiviral vector that expresses an shRNA that knocks down SVA-lncRNA AK057321 expression
202835SVA-lncRNA AK057321 shRNA 1A scramble controlLentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1A
202836pLVX-SVA-lncRNA AK057321 shRNA 1SLentiviral vector that expresses an shRNA that knocks down SVA-lncRNA AK057321 expression
202837SVA-lncRNA AK057321 shRNA 1S scrambleLentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1S
202838pLVX-SVA-lncRNA AK057321 shRNA 2SLentiviral vector that expresses an shRNA that knocks down SVA-lncRNA AK057321 expression
202839SVA-lncRNA AK057321 shRNA 2S scrambleLentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 2S
202840TetO-CDK5RAP2Doxycycline inducible expression of CDK5RAP2 cDNA

Antibodies from Article