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Addgene
nucGEMs probe the biophysical properties of the nucleoplasm
Shu T et al.
bioRxiv 2022
Article

Plasmids from Article

ID Plasmid Purpose
203648pUBC-Pfv-Sapphire-IRES-H2B-mCherryMammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.
203649pUBC-Pfv-SapphireMammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter.
203650TetCMV-Pfv-SapphireMammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the doxycycline-inducible promoter.
203651pUBC-Pfv-Sapphire_PUROMammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.
203652pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherryMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.
203653pUBC-SV40NLS-Pfv-SapphireMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.
203654pUBC-SV40NLS-Pfv-Sapphire-PUROMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.
203655pRS305-LEU2-pINO4-SV40NLS-PfV-GS-SapphireYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.
203656pRS305-LEU2-pINO4-SV40NLS-PfV-GS-yEmCherryYeast integrative vector for the expression of the yEmCherry-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.
203657pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3Yeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.
203658pRS403-HIS3-pINO4-SV40NLS-PfV-GS-SapphireYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.
203659pINO4-Pfv-Sapphire-HOYeast vector encoding donor DNA for integration of 40nm GEMs into the HO locus.
203660pINO4-NLS-Pfv-Sapphire-HOYeast vector encoding donor DNA for integration of nuclear 40nm GEMs into the HO locus.

Antibodies from Article